蚜虫传毒专化性与病毒附着蛋白的关系  

Relationship of Virus Attachment Site and Specificity of Aphid Transmission

作　　者：吴云锋[1] 周广和 柳树斌 

机构地区：[1]西北农业大学植保系,陕西杨陵712100 [2]中国农科院植保所,北京100093 [3]韩城市植保站

出　　处：《西北农业学报》1999年第4期5-7,共3页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家攀登计划;国家863青年基金;植物病虫害生物国家重点实验室基金

摘　　要：用免疫荧光标记研究发现．挑蚜下颚口针有非持久性病毒WMMV－2和TEV的病毒附着位点，距口针尖端45～60μm，结合区全长约45～55μm，病毒附着蛋白含有α-D-甘露糖残基。用O．2％DOC溶液处理1h可破坏病毒附着位点。用0．2％DOC处理蚜虫后，分别构取PAV和GPV，结果禾谷缢管蚜传播GPv和PAv的能力显著降低，麦长管蚜和麦二叉蚜则不能传播GPV和PAV。从禾谷经管蚜虫体提取的RP1和RP2分子量分别约为83kD和57kD。从桃蚜口针中分离到一种相关组分，其分子量为76kD。Immuno--FITC labels were used to determine the location of Virus attachment site(VAS)in the maxillae of Myzus persicae and Aphis gossypii,which can acquire watermelon mosalc virus 2 (WM-MV- 2), tobacco etch virus (TEV) and cucumber mosaic virus T (T- CMV ). Immunofluorescence mi-croscopical observations revealed that VAS is about 45 μm long, located at 40~50 μm from tips of maxil-iae. VAS contain α-D-mannosyl residues. Aphid stylets, which were conducted by 0. 2％ deoxycholate(DOC), Myzus persicae and Aphis gossypii lost VAS for transmission viruses. VAP from Myzus persicaewith molecular weight is about 76 kD. Rhopalosiphum padi can transmit GAV,and lower transmission ofGPV and PAV, Sitobion avenae and Schizaphis graminum can not transmit GPV and PAV. Proteins puri-fied from hindgut membrane and body of R. padi with molecular weights are about 83 kD and 57 kD.

关 键 词：蚜传病毒 病毒附着蛋白 病毒 蚜虫 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]