我国棉花黄萎病菌基于SSR的遗传多样性分析  

Genetic Diversity of Verticillium dahliae Populations of Cotton in China Based on SSR Analysis

作　　者：尹志新[1] 朱荷琴[1] 李志芳[1] 冯自力[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《棉花学报》2011年第4期369-378,共10页Cotton Science

基　　金：国家公益性农业科研专项(3-19);科技基础性工作专项(SB2007FY027);国家重大专项重点课题(2009ZX08011-26B)

摘　　要：为了对棉花黄萎病菌群体的遗传多样性进行研究,以棉花黄萎病菌基因组DNA为模板,用双因素和单因素水平优化的方法对SSR反应体系的重要参数进行摸索和优化,建立了最适反应体系。从300对引物中筛选出13对多态性较高的引物,并对来自我国12个省96个县(市)的170个棉花黄萎病菌株进行SSR分析。SSR图谱的聚类分析结果将供试菌株划分为2个群体:群体Ⅰ有26个菌株,其中18个为新疆菌株,占69.23%;群体Ⅱ有144个菌株,其中138个为内地菌株,占95.83%。新疆菌株与其它省份的菌株存在明显差异,SSR指纹图谱与菌株地理来源存在显著的相关性,而与菌株致病力强弱没有表现出相关性,但弱致病力菌株可能更容易发生遗传变异。In order to study genetic diversity of Verticillium dahliae populations isolated from cotton,using genome DNA of Verticillium dahliae as template,important parameters of the SSR reaction system were optimized through single-factor and double-factor analysis,and the optimal SSR reaction system for Verticillium dahliae was establishied.13 pairs of polymorphic higher SSR primers chosen from 300 primers were used to amplify genome DNA of 170 tested isolates of Verticillium dahliae from 96 counties（cities） of 12 provinces in China.In SSR profiles of the cluster analysis,these isolates were classified into two groups： GroupⅠincluded 26 isolates,18 isolates of this group were from Xinjiang which accouned for 69.23%;GroupⅡincluded 144 isolates,138 isolates of this group were from the inland which accounted for 95.83%.So there was significant difference between the isolates in Xinjiang and other provinces.SSR fingerprinting exhibited that there was a significant correlation with geographical origin of isolates,no correlation with the pathogenicity,but weak virulence isolates may be more prone to genetic variation.

关 键 词：棉花 大丽轮枝菌 SSR 遗传多样性分析 非加权组平均法 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]