雌激素在大鼠子宫内膜异位症模型建立中的作用  

The role of estrogen during establishment of endometriosis model in rat

作　　者：肖成明[1] 曹丹[1] 张红[1] 哈春芳[2] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏医科大学附属医院,宁夏银川750004

出　　处：《宁夏医学杂志》2011年第7期582-584,共3页Ningxia Medical Journal

基　　金：宁夏科技攻关项目(KGX130906);宁夏医科大学特殊人才资助项目(XT200914)

摘　　要：目的在大鼠内异症模型前后给予雌激素统一动情期,探讨雌激素在建立大鼠子宫内膜异位症(EMS)模型中的作用。方法 40只大鼠平均分成四组,A组建模前给雌激素建模后不给雌激素,B组建模前不给激素建模后给雌激素,C组建模前后均给雌激素,D组建模前后均未给激素,应用自体移植法建立大鼠EMS模型,观察建模前后大鼠子宫直径、异位囊肿的体积,免疫组化法测定正常子宫及异位内膜中OPN、MMP-9的表达。结果建模前A组大鼠子宫直径大于B组直径(P<0.05);二次开腹时D组子宫直径最小与另外三组比较差异有统计意义,C组子宫直径最大,与A、B组比较差异有统计学意义,A组与B组间比较差异无统计学意义(P>0.05);C组异位囊肿体积最大,与B、D组比较差异有统计学意义,与A组比较差异无统计学意义;D组囊肿体积最小,与A、C两组比较差异有统计学意义(P<0.05),与B组比较无统计学意义(P>0.05);成模后异位内膜中MMP-9的表达,C组高于A、B、D三组(P<0.05),而后三组间相互比较差异无统计学意义;成模后异位内膜中OPN的表达,D组低于A、B、C三组表达,但A、B、C两组间比较差异无统计学意义;A、B、C、D四组建模成功率差异无统计学意义。结论雌激素能降低建模时手术操作的难度,提高异位内膜中OPN、MMP-9的表达但不影响总建模率。Objective To study the role of estrogen during establishment of endometriosis model in rat.Methods 40 rats were randomly divided into four groups： Group A,estrogen was given before making models;Group B,estrogen was given after making models;Group C,estrogen was given during the progress of making models;Group D,the normal control group.The rat endometriosis model was established by using john’s method and the diameters of rat uterus and the size of ectopic cyst in models was observed.The expressions of OPN and MMP-9 was detected by using immunohistochemical method in normal and ectopic endometriums.Results The uterine diameter of group A was larger than that in group B before making models（P〈0.05）.The uterine diameter of group D was smaller than that in other groups.The uterine diameter in group C was larger when second operation.Comparing with group B and D,the volume of cysts in group C were bigger,but showed the same volume in group A,and compared to group A and C,the volume of cysts in group D were significantly small（P〈0.05）,but showed the same volume in group B（P〉0.05）.The expression of MMP-9 in group C was significantly higher than that in group A,B and D,which showed the same expression.The expression of OPN in group D was significantly higher than that in group A,B and C,which showed the same expression.There was similar success rate of model establishment in four groups.Conclusion Estrogen is effective in the endometriosis models in rat,but don’t affect modeling rate.

关 键 词：雌激素 子宫内膜异位症 OPN MMP-9 大鼠 

分 类 号：R711.71[医药卫生—妇产科学]