镧、钆、镱三种稀土离子诱导背根神经元凋亡及膜上钾电流的比较研究  

A Comparative Study on La^(3+),Gd^(3+) and Yb^(3+) Modulation of Potassium Currents and Induction Apoptosis in Dorsal Root Ganglion Neurons

作　　者：杜正清[1] 杨频[2] 

机构地区：[1]山西财经大学环境经济学院,山西太原030006 [2]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《中国稀土学报》2011年第4期496-503,共8页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金重大项目(20637010);山西省青年学术带头人支持计划资助

摘　　要：运用流式细胞仪和膜片钳技术研究La3+,Gd3+,Yb3+三种稀土离子诱导大鼠背根神经元DRG凋亡以及膜上钾离子通道的影响。结果表明:10,100,1000μmol.L-1LaCl3和GdCl3处理DRG神经元96 h,细胞不出现凋亡;YbCl3处理96 h,细胞的凋亡率明显递增。胞外La3+,Gd3+,Yb3+抑制瞬间外向钾电流IA,使IA的激活和失活过程都显著右移,抑制和右移的程度La3+最弱,Gd3+次之,Yb3+最强;胞内的La3+,Gd3+,Yb3+抑制延迟整流钾电流IK,抑制的程度也呈增长趋势。稀土离子在胞内外的结合位点不同;Yb3+较La3+和Gd3+的神经毒性强。Apoptosis of rat dorsal root ganglion（DRG） neurons induced by La3＋,Gd3＋ and Yb3＋,as well as their influence on K＋-channels were studied by flow cytometry and whole-cell patch clamp method.The results showed that the apoptosis rate did not change after the DRG neurons were treated with LaCl3 and GdCl3 of 10,100 and 1000 μmol·L-1 for 96 h,while the apoptosis rate with three concentration groups of YbCl3 increased significant.The La3＋,Gd3＋ and Yb3＋ of 100 μmol·L-1 in the extracellular solution played a dominant role in inhibiting the transient outward potassium current（IA）.Both activation and inactivation of IA shifted significantly to the right,where La3＋ had the weakest effect while Yb3＋ had the strongest.The La3＋,Gd3＋ and Yb3＋ in the intracellular solution,however,only inhibited the rectifier potassium current while the effect of inhibition also displayed augmented tendency.In conclusion,Yb3＋ had relatively more serious neurotoxicity than La3＋ and Gd3＋.

关 键 词：DRG细胞 镧离子 钆离子 镱离子 凋亡 钾电流 

分 类 号：Q28[生物学—细胞生物学] O614[理学—无机化学]