西藏砂生槐丛生芽的诱导  

Induction of Adventitious Bud on Sophora moorcroftiana in Tibet

作　　者：杨爽[1] 方江平[1] 唐佳[1] 王勇 

机构地区：[1]西藏农牧学院生物技术中心,林芝860000 [2]西藏林芝地区农牧局,林芝860000

出　　处：《吉林农业大学学报》2011年第4期381-384,共4页Journal of Jilin Agricultural University

基　　金：国家林业局林业科技推广示范项目(XZ2009001);国家农业科技成果转化资金项目(2009GB2F400356)

摘　　要：以西藏砂生槐无菌实生苗为材料，研究了不同外植体、基本培养基、细胞分裂素种类及浓度、pH对其丛生芽诱导的影响。结果表明：以子叶节为外植体，接种于pH6．0—6．5的MS＋6-BA2．0mg／L＋NAA0．2mg／L培养基上，丛生芽的诱导效果最好。Hypocotyl explants induced adventitious buds from Sophora moorcrofiiana seedling by adopting shoot tips, stems and cotyledon nodes. Different explants, basal substrates, kinds and concentrations of cytokinin and pH value were involved. The results showed that the induction effects of Multiple Shoots were the best in the basic medium at the conditions of pll 6.0--6.5 with MS ＋ 6-BA 2. 0 mg/L ＋ NAA 0.2 mg/L.

关 键 词：西藏砂生槐 丛生芽 诱导率 

分 类 号：S723.139[农业科学—林木遗传育种] S792.26[农业科学—林学]