梨SRAP体系的优化及其在杂交后代中的分离方式  

Optimization of Reaction System for SRAP Molecular Marker in Pear and Segregation Patterns in Hybrid Progenies

作　　者：郭印山[1] 赵玉辉[1] 李坤[1] 郭修武[1] 王家珍[2] 李俊才[2] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110866 [2]辽宁省果树科学研究所,熊岳115009

出　　处：《吉林农业大学学报》2011年第4期390-394,共5页Journal of Jilin Agricultural University

基　　金：辽宁省重大科技攻关项目(200820403);辽宁省教育厅科研基金项目(L2010492);沈阳农业大学青年教师基金项目(20092005)

摘　　要：采用正交设计建立并优化了梨的SRAP-PCR分子标记技术体系,在20μL的反应体系中含有DNA15 ng/mL,Primer 0.5μmol/L,dNTPs 0.1 mmol/L,Mg2+2 mmol/L,TaqDNA聚合酶1.5 U。应用20对引物组合对SRAP标记在"红巴梨×南果梨"F1群体的多态性和分离方式进行了研究。结果表明:SRAP标记在该群体中具有较高的多态性,分离位点占总位点数的44.59%。其中,符合孟德尔分离位点110个,占分离位点数的83.33%;偏离孟德尔分离比例位点22个,占分离位点数的16.67%;异常分离位点8个,占总位点数的2.55%。By the method of orthogonal design, the reaction system for SRAP- PCR molecular marker in pear was established and optimized, 15 ng/mL DNA, 0.5μmol/L Primer, 0.1 mmol/L dNTP, 2 mmol/ L Mg2＋ , 1.5 U Taq DNA polymerase in 20 μL reaction system. The polymorphism and the segregation patterns of the SRAP markers in the Fl progenies from two pear cultivars （Red Bartllet x Nanguo pear） were studied using 20 primer combinations. The results showed that the polymorphism of the SRAP markers in the FI population was high, and the frequency of the segregation loci was 44.59 %. The segregation patterns included Mendelian, deviation from Mendelian and new segregation loci, and their frequency and number were 83.33 %, 110 ; 16.67 %, 22 ; 2.55 %, 8 respectively. All the above could provide some theoretical basis for the further work of the construction of pear genetic map and relevant study.

关 键 词：梨 分子标记 SRAP 分离方式 

分 类 号：S661.2[农业科学—果树学]