SPRN转基因小鼠感染Scraipe鼠适应性病毒后动物行为与脑组织形态比较  

Behavior and Histomorphology of SPRN Infected with Adaptability Virus of Scraipe Mice

作　　者：王东旭[1,2] 林超[1] 万家余[2] 潘朋朋[2] 李莎[1] 冯立文[3] 高宏伟[2] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]军事医学科学院军事兽医研究所,长春130062 [3]吉林大学农学部,长春130062

出　　处：《吉林农业大学学报》2011年第4期433-438,共6页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(30972197;31072148)

摘　　要：通过建立Scraipe鼠适应性病毒感染Tg（sprn—B）／C57BL／6转基因小鼠与C57BL／6野生型小鼠，观察鼠行为学及脑组织形态学的病理变化，探讨Shadoo蛋白在感染过程中对小鼠学习记忆能力及中枢神经系统的影响。结果显示在Morris水迷宫定位航行中，4～7d转基因感染组与野生型感染组的逃避潜伏期差异极显著（P〈0．01）；空间探索试验中，转基因感染组与野生型感染组的第1次穿越平台时间及穿越平台次数羞异极显著（P〈0．01）。HE染色观察鼠脑切片发现，转基因感染组与野生型感染组相比，海马、皮质、小脑空泡数量略少，海马区锥体层细胞排列比较紧密，细胞轮廓比较完整，小脑蒲肯野氏细胞清晰明显。Through establishing the animal models of both Tg（sprn- B）/C57BL/6 transgenic mice and C57BL/6 wild-type mice infected with adaptability virus of Scraipe mice, mouse behavior and pathological changes of histomorphology were observed and the effects of Shadoo protein on learning ability and central nervous system of mice in the process of infection were studied. The results show that escape la- tency of transgenic and wild-type infection groups were significantly different （ P 〈 0.01 ） in the Morris water maze place navigation at 4, 5, 6, 7 days. In the spatial probe test, time of first crossing platform and crossing times of transgenic and wild-type infection groups were significantly different （P 〈 0.01 ）. Compared to wild-type infection group, infection group was less, hippocampal integral, and cerebellar purkinje cells the number of hippocampus, cortex and cerebellum of transgenic pyramidal layer cells were closely arranged and their outline was were clear by HE staining.

关 键 词：Scraipe鼠适应性病毒 SPRN基因 MORRIS水迷宫 组织形态学 

分 类 号：S852.21[农业科学—基础兽医学]