半饥饿对转基因鲤生长和性腺发育的影响  

Effects of Semi-Starvation on the Growth and Gonad Development of Transgenic Carps

作　　者：刘春雷[1,2] 徐丽华[1,2] 梁利群[1] 刘金亮[1,2] 常玉梅[1] 

机构地区：[1]中国水产科学院黑龙江水产研究所,哈尔滨150070 [2]上海海洋大学水产与生命学院,上海200090

出　　处：《吉林农业大学学报》2011年第4期450-454,463,共6页Journal of Jilin Agricultural University

基　　金：国家高技术研究发展计划项目(2007AA10Z186)

摘　　要：为研究转大麻哈鱼(Oncorhynchus keta)生长激素基因鲤(转基因鲤)在不同食物含量条件下的生长竞争(主要是生长、生殖),设定正常投饲组和半饥饿试验组,每组饲养池大小相同、混养的转基因鲤和对照鲤初始总体质量相等。连续3个月定期采样后,从形态学和组织学研究转基因鲤和对照鲤(Cyprinus carpio)的生长状况、饲料占有情况和性腺发育状况。结果表明:正常投饲组的转基因鲤和对照鲤体质量相对增长率分别为127.9%和70.6%,而半饥饿试验组的转基因鲤、对照鲤的体质量相对增长率分别是72.3%和52.2%,表明无论在饱食还是在半饥饿情况下,转基因鲤均显示出快速生长的优势;2组试验鱼的性腺发育进程无明显差异(P>0.05),但在部分月份转基因鲤和对照雌、雄鲤的相对性腺质量差异显著(P<0.05),无论在饱食还是在半饥饿情况下,转基因鲤的性腺发育情况略好于对照鲤。In order to study the growth competition and gonad development of transgenic carps of trans- ferred salmon growth hormone gene （thereafter referred to transgenic carp） and wild carps in semi-starvation condition, the experimental fish were divided into groups A and B with the same total weight, in which contained transgenic carps and wild carps with equal gross weight. Group A was fed with enough food and group B was fed with half of the former. Sampled in consecutive three months, the growth performance and gonad development were compared between transgenie carps and its counterpart in two groups. As a result, the relative growth rates of body weight were 127.9% and 70.6% individually in two carps from group A, the same values in two carps of group B were 72.3 % and 52.2 %, respectively, demonstrating that the transgenic carps had the advantage of fast growth. In addition, there was no differ- ence during the gonad development in two groups （ P 〉 0.05）, however, the value of relative gonad weight of transgenic carp was remarkably higher than that of the control in certain month （ P 〈 0.05）.Overall, the gonad of transgenic carp developed better than the control no matter where group A or group B came from.

关 键 词：转基因鲤 半饥饿 大麻哈鱼生长激素基因 生长 性腺发育 

分 类 号：S965.116[农业科学—水产养殖]