导流杂交基因芯片法和聚合酶链反应-反向点杂交法检测人乳头瘤状病毒亚型的比较  

Comparison between flow-through hybridization and gene chip and polymerase chain reaction-reverse dot blot hybridization for detecting human papilloma virus subtype

作　　者：毛晓丹[1] 孙蓬明[1] 林芬[1] 宋一一[1] 

机构地区：[1]福建省妇幼保健院,福州350001

出　　处：《海峡预防医学杂志》2011年第3期4-6,共3页Strait Journal of Preventive Medicine

基　　金：福建省医学创新课题(No.2009-CXB-33)

摘　　要：目的探讨导流杂交基因芯片法和聚合酶链反应-反向点杂交法检测人乳头瘤状病毒(HPV)亚型的差异。方法随机选取HPV标本50例,分别用导流杂交基因芯片法和聚合酶链反应-反向点杂交法检测HPV亚型,比较检测结果,结果不相符或部分相符的标本采用基因测序方法验证。结果两种方法检测结果,19例阴性标本和25例阳性标本完全相符,共44例检测标本结果完全相符,绝对符合率88%(44/50);4例检测结果部分相符,相对符合率96%(48/50),4%不相符(2/50)。结论导流杂交基因芯片法和聚合酶链式反应-反向点杂交法检测HPV-DNA型别检出结果类似,均能快速、准确地检测HPV感染。Objection To explore the differences between flow-through hybridization and gene chip and polymerase chain reaction-reverse dot blot hybridization（PCR-RDB）for detecting human papilloma virus（HPV） subtype.Method Totally 50 samples were collected randomly to detect HPV by flow-through hybridization and gene chip and PCR-RDB method independently.Comparing the results,the different results or partly alike results were identified by genetic sequencing method.Results Totally 44 samples were coincident（19 negative and 25 positive samples）,absolute match rate was 88%（44/50）,four samples were partial alike,relative match rate was 96%（48/50）,4% samples were different（2/50） by the two methods.Conclusion The results of flow-through hybridization and gene chip and PCR-RDB for detecting HPV subtype are similar.HPV infection can be detected quickly and accurately by the two methods.

关 键 词：人乳头瘤状病毒亚型 宫颈癌 导流杂交基因芯片技术 聚合酶链式反应-反向点杂交法 

分 类 号：R373[医药卫生—病原生物学] R737.33[医药卫生—基础医学]