运动性免疫抑制中胸腺IL-7和TGF-β1应答性特征  

Responsive Characteristics of IL-7 and TGF-β1 in thymus during Exercise-induced Immunosuppression

作　　者：张琳[1] 郝选明[1] 

机构地区：[1]华南师范大学,广东广州510006

出　　处：《体育学刊》2011年第4期137-140,共4页Journal of Physical Education

基　　金：广东省自然科学基金(9151063101000059);第49批中国博士后科学基金面上资助项目(20110490909)

摘　　要：通过胸腺细胞因子IL-7和TGF-β1及其mRNA进行研究,探讨运动性免疫抑制发生发展过程中胸腺细胞发育的调节机制。将128只8周龄雄性SD大鼠随机分为运动组和对照组,运动组进行递增负荷跑台训练6周,每周6次,周日休息,每次30 min。第1周负荷为10 m.min-1,第2周负荷为20 m.min-1,此后每周增加5 m.min-1,至6周时达40 m.min-1。分别于第0、2、4、6周利用ELISA和FQ-RT-PCR技术测相对安静状态、运动后即刻和运动后3 h IL-7和TGF-β1及其mRNA的表达水平。结果显示:6周递增负荷跑台运动过程中,IL-7和TGF-β1呈现几乎相反的应答性变化:IL-7及mRNA第0周、第2周末运动后明显降低,恢复3 h后升高,呈"V"型应答曲线;第4周末,运动前后没有显著性变化;第6周末,在运动后持续下降。TGF-β1在各周呈现倒"V"型变化,TGF-β1 mRNA对负荷初次应答时运动前后没有明显变化,其它各周呈倒"V"型变化。以上结果说明运动性免疫抑制发生发展中胸腺IL-7下降和TGF-β1升高可能导致胸腺微环境紊乱,从而影响胸腺细胞发育。To investigate the regulatory mechanism of thymocyte development during exercise-induced immuno-suppression,IL-7 and TGF-β1 as well as their mRNA of rats in thymus were tested.A total of 128 Sprague–Dawle male rats,aged 8 weeks,were divided into Control Group and Exercise Group.Incremental ergomitry Exercise pro-tocol（10 m？min-1 in week1,20 min week2,5 m？min-1 increased per week,successive 6 weeks,6 d per week,30 min per day）.Samples were taken in week 0,week 2,week 4 and week 6 before exercise,just after exercise and 3 h after exercise,respectively.IL-7 and TGF-β1 as well as their mRNA of rats in thymus were calculated by ELISA and FQ-RT-PCR.Results：1） During six weeks incremental exercise： The responsive tendency of IL-7 and its mRNA presented the shape of ＂V＂ letter during week 0 and week2,while there was no distinguished changes during week4,and last decreased in week6.TGF-β1 and its mRNA presented that of inversed ＂V＂ letter during every week,except its mRNA was no prominent change in week0.Conclusion： The decrease of IL-7 and increase of TGF-β1 during Exercise-induced immunosuppression could be resulted the disorder of thymic microenvironment which effected thymocyte development.

关 键 词：运动生物化学 运动性免疫抑制 白介素-7 转化生长因子-Β 胸腺 应答性特征 

分 类 号：G804.7[文化科学—运动人体科学]