脓毒症大鼠的白细胞和血小板的变化规律及发生机制  

The changes of leukocytes and blood platelets in septic rats and its mechanisms

作　　者：李杰[1] 王东强[2] 田永超[3] 付鹏亮[3] 李双[3] 李志军[2] 

机构地区：[1]天津中医药大学附属南开中医院,天津市南开区中医医院,天津300102 [2]天津市第一中心医院,天津300192 [3]天津中医药大学,天津300193

出　　处：《西安交通大学学报（医学版）》2011年第4期421-423,461,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：2010年天津市应用基础与前沿技术重点项目(No.10JCZDJC18900)~~

摘　　要：目的观察脓毒症大鼠白细胞(WBC)和血小板(PLT)的变化规律并探讨其可能的发生机制。方法 72只Wistar大鼠随机分为3组:对照组(n=8)、假手术组(n=8)、模型组(n=56)。模型组又分为7个亚组(每个亚组n=8);采用盲肠结扎穿孔术(CLP)复制脓毒症大鼠模型,观察每组大鼠一般情况、肺组织病理及WBC、PLT的变化。结果模型组大鼠病态表现明显,WBC、PLT的变化在假手术组与对照组间比较,差异无统计学意义(P>0.05),而模型组与对照组和假手术组间比较差异均有统计学意义(P<0.05或P<0.01),模型组WBC、PLT的整体变化趋势均表现为先明显降低后缓慢回升。结论脓毒症大鼠WBC、PLT的异常变化可能与脓毒症时骨髓抑制、细胞趋化聚集或免疫功能紊乱的发生有关。Objective To observe the changes of leukocytes and blood platelets in septic rats and explore the possible mechanisms.Methods Totally 72 Wistar rats were randomly divided into three groups： control（n=8）,sham operation（n=8） and model（n=56） groups.Then the model group was randomly divided into seven sub-groups with 8 rats in each.The septic rat models were established by cecum ligation perforation.The changes in general conditions,lung pathology,leukocytes and blood platelets were observed in rats in each group.Results The model group rats showed apparent morbid state.There were no significant differences in the changes of leukocytes and blood platelets between the sham group and the control group（P〉0.05）.However,the model group differed significantly from the control and sham groups（P〈0.05 or P〈0.01）.The overall trend of leukocyte and blood platelet changes in the model group was from obvious decrease to slow rise.Conclusion The abnormal changes of leukocytes and blood platelets in septic rats may be related to sepsis-caused bone marrow suppression,cellular chemotaxis and aggregation or immunity disorder.

关 键 词：脓毒症 白细胞 血小板 骨髓抑制 细胞趋化聚集 免疫功能紊乱 

分 类 号：R459.7[医药卫生—急诊医学]