急性肺损伤大鼠肺组织TOLL样受体4及CD14mRNA的表达  

Expression of TLR4 and CD14 mRNA in rats with acute lung injury induced by lipopolysaccharide

作　　者：杨爱东[1] 李文雯[1] 王利霞[1] 汪东颖[1] 吴中华[1] 苏中昊[1] 庞慧芳[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中国实验动物学报》2011年第3期216-219,278,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家中医药管理局重点学科项目;上海市重点学科建设项目(S30301);上海市教委高校高水平特色发展项目[沪教委财(2005)81号];上海市高等学校科学技术发展基金项目(04CB17);上海中医药大学名师研究室项目(Z1008210);上海高校选拔培养优秀青年教师科研专项基金(P13908)

摘　　要：目的探讨内毒素致急性肺损伤大鼠肺组织TOLL样受体4及CD14 mRNA表达的变化。方法 24只雄性Wistar大鼠随机分为2组:对照组、LPS模型组,每组再分为4 h和8 h两个亚组。尾静脉注射脂多糖(LPS)(10 mg/kg)建立大鼠急性肺损伤模型。检测大鼠动脉血气、肺体指数,实时荧光定量PCR测定肺组织TOLL样受体4及CD14 mRNA的表达,并观察肺组织病理变化。结果与对照组相比,模型组4 h和8 h时大鼠肺组织中的TLR4及CD14 mRNA表达均显著增高(P<0.05或P<0.01)。病理学观察显示,模型组大鼠肺组织出现出血及坏死。结论内毒素致急性肺损伤的发病机制可能与TLR4及CD14 mRNA的表达升高有关。Objective To investigate the changes of TLR4 and CD14 mRNA expression in the rats with acute lung injury（ALI） induced by lipopolysaccharide（LPS）.Methods Twenty-four male Wistar rats were randomly divided into two groups： normal control group and LPS model group.Each group had two subgroups,4 hours and 8 hours after LPS injection.The ALI model was established by intravenous injection of LPS（10 mg/kg） through the tail vein.The arterial blood gas graph（ABGG） and lung-body index were analyzed.The expressions of TLR4 and CD14 mRNA were measured by real-time fluorescent polymerase chain reaction（PCR）,while the histopathology of the lung injury was observed by light microscopy.Results Compared with the normal group,the expressions of TLR4 and CD14 mRNA in LPS model group were significantly increased at 4 hours and 8 hours（P0.05 or P0.01）.Light microscopic observation indicated that there were pulmonary hemorrhage and necrosis in the model group.Conclusion The increase of TLR4 and CD14 mRNA expressions may be involved in the pathogenetic mechanism of acute lung injury caused by lipopolysaccharide.

关 键 词：脂多糖 急性肺损伤 TOLL样受体4 CD14 

分 类 号：Q95-33[生物学—动物学]