可降解组织工程血管支架成功用于犬股动脉移植  

Successful application of biodegradable vascular scaffolds in the dog femoral artery transplantation

作　　者：武欣[1] 谷涌泉[1] 段红永[1] 陈兵[1] 李建新[1] 吴英锋[1] 张淑文[1] 汪忠镐[1] 张建[1] 

机构地区：[1]首都医科大学宣武医院血管外科,北京100053

出　　处：《中国实验动物学报》2011年第3期250-254,279,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2006AA02A134);北京市自然科学基金项目(项目编号:5082007和2103049)资助

摘　　要：目的采用可降解的聚己内酯接枝肝素材料,负荷b-FGF(碱性成纤维细胞生长因子),体外构建的小口径组织工程血管,完成犬的股动脉移植动物实验。方法利用可降解的聚己内酯接枝肝素材料,电纺丝技术制备组织工程血管支架,并对支架负荷b-FGF生长因子,并进行材料的内皮细胞粘附实验。将体外构建的小口径组织工程血管,完成犬的股动脉移植动物实验,观察通畅率和移植术后组织工程血管的改变。结果可降解聚己内酯接枝肝素材料支架,负荷细胞生长因子(b-FGF),利于内皮细胞粘附。构建的组织工程血管进行体外动物实验构建,3个月移植物通畅率好,移植后取材,有新生内膜迁移和胶原纤维浸入。结论利用可降解聚己内酯接枝肝素材料构建小口径支架,初步符合构建组织工程血管支架的要求。Objective To investigate the feasibility of heparin-bonded biodegradable polycaprolactone（PCL） materials in animal femoral artery transplantation.Methods Heparin-bonded biodegradable PCL materials loaded with basic fibroblast growth factor（b-FGF） were used as vascular tissue engineering scaffold,and small-diameter tubular scaffolds for tissue engineering blood vessel were constructed using electrospinning technique.Endothelial cell adhesion experiment was conducted to evaluate the adhesion of endothelial cells on the scaffords.The small-diameter tubular scaffold was used to femoral artery transplantation in dogs.The patency rate and histological changes in the tissue engineering vascular graft were observed and evaluated.Results The use of heparin-bonded biodegradable PCL materials to construct vascular scaffolds was successful.The cell adhesion experiment showed a better endothelial cell adhesion to the biodegradable scaffold loaded with basic fibroblast growth factor（b-FGF）.Three-month graft patency rate of the dog femoral artery transplantation was high（83.3%,5/6）.Histological examination showed migration of neointimal endotheliual cells and infiltration of fibroblasts.Conclusions Our results preliminarily show that heparin-bonded biodegradable polycaprolactone materials can be ued as small-diameter vascular tissue engineering scaffold,and meet the tissue engineering requirements.

关 键 词：可降解聚己内酯材料 血管支架 组织工程 抗凝 股动脉移植 通畅性 犬 

分 类 号：Q95-33[生物学—动物学]