聚乳酸聚乙醇酸膜、聚β羟基丁酯膜和胶原膜三种材料的相容性探讨  

Preliminary study of poly(lactic acid-glycolic acid),poly-β-hydroxybutyrate and collagen membrane as scaffold in tissue-engineered vessels

作　　者：苏立伟[1] 李霞[2] 赵东锷[1] 马毅[3] 牛中喜[1] 张成伟[1] 李玮[1] 

机构地区：[1]武警总医院胸外科,北京100069 [2]武警总医院药剂科,北京100069 [3]武警总医院核磁共振科,北京100069

出　　处：《中国实验动物学报》2011年第3期255-258,F0003,共5页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的评价聚乳酸聚乙醇酸膜、聚β羟基丁酯膜和胶原膜的结构、生物相容性及其在组织工程血管中的应用前景。方法 HE、胶原染色,扫描电镜观察材料的结构。新西兰兔15只皮下植入材料,于4、6、8和12周取出观察其组织反应和降解情况。将犬股动脉间质细胞种植于聚乳酸聚乙醇酸膜、胶原膜上,观察其形态。结果聚乳酸聚乙醇酸膜、聚β羟基丁酯和胶原膜的孔径分别为179、13、107μm,3种材料组织相容性好,局部无组织坏死和脓肿形成。早期以中性粒细胞和嗜酸性粒细胞浸润为主,后期以淋巴细胞浸润为主。聚乳酸聚乙醇酸膜于8周基本吸收,胶原膜12周吸收,聚β羟基丁酯膜12周未见明显降解吸收。细胞种植实验显示细胞在聚乳酸聚乙醇酸膜和胶原膜表面生长良好。结论聚乳酸聚乙醇酸膜和胶原膜在材料结构、组织及细胞相容性和降解率方面符合组织工程血管支架材料的要求。聚β羟基丁酯膜在材料结构、降解率和细胞相容性方面尚需改进。Objective To evaluate the structural characteristics,biodegradation and biocompatibility of collagen,poly（lactic acid-glycolic acid） and poly-β-hydroxybutyrate（PHB） membranes and their future application in tissue-engineered blood vessels.Methods Structural characteristics of the materials were observed by light microscopy using HE and picric-sirius red staining for collagen,and scanning electron microscopy.All materials were implanted subcutaneously in rabbits and extirpated at 4,6,8,12 weeks after inplantation,respectively,to study their biocompatibility and biodegradability.Interstitial cells of canine femoral artery were seeded on the PLGA and collagen membranes to evaluate their biocompatibility.Results The pore diameters of the collagen,PLGA and PHB membranes were 107 μm,179 μm and 13 μm,respectively.All materials were well tolerated by the tissue and no abscess or tissue necrosis was observed.There were neutrophil and eosinophil infiltration in the early stage and lymphocyte infiltartion in the later stage.Collagen and PLGA membranes were completely degraded at the end of 12 weeks.No degradation of PHB was observed at the end of 12 weeks.Interstitial cells grew well on the PLGA and collagen membranes.Conclusions Collagen and PLGA membranes meet the demands of scalffold of tissue-engineered vessel regarding thier structural characteristics,biocompatibility and biodegradation.

关 键 词：聚乳酸聚乙醇酸 聚β羟基丁酯 胶原 血管 

分 类 号：Q95-33[生物学—动物学]