WIP1对小鼠B细胞及T细胞发育的影响  

The Role of WIP1 in Mouse B cell and T cell Development

作　　者：陈陟阳[1] 张俊伶[1] 马小茗[1] 易微微[1] 陈显达[1] 石桂英[1] 鞠振宇[1] 

机构地区：[1]中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国比较医学杂志》2011年第6期52-59,共8页Chinese Journal of Comparative Medicine

基　　金：实验血液学国家重点实验室开放课题[ZK10-05]

摘　　要：目的研究WIP1基因对小鼠骨髓B细胞发育及胸腺T细胞发育的影响。方法流式细胞术测定小鼠骨髓B细胞及胸腺T细胞发育中各阶段的细胞比例。结果虽然WIP1缺失小鼠骨髓B细胞发育各阶段比例正常,但骨髓总体B细胞比例下降;WIP1基因敲除小鼠胸腺发育障碍,CD8/CD4双阴性细胞比例增高,CD8/CD4双阳性细胞比例降低。结论 WIP1基因在小鼠骨髓B细胞及胸腺T细胞的发育过程中起重要作用。Objective To study the role of WIP1 gene in mouse B cell and T cell development.Methods The proportion of the B cell in bone marrow and T cell in the thymus were determined by the flow cytometry.Results Although the proportion of different subsets in B cell development was normal,the total number of B cell in bone number was decreased.WIP1 gene knockout mice had defect in thymus development,the proportion of CD8/CD4 double negative cells was increased,but the CD8/CD4 double positive cells was decreased.Conclusion WIP1 gene plays an important role in the development of B cell and T cell.

关 键 词：WIP1 基因敲除小鼠 B细胞 T细胞 

分 类 号：R349.15[医药卫生—基础医学] R332