硫化氢对心肺复苏后大鼠心肌缺氧诱导因子-α的影响  

The effects of hydrogen sulfide on expression of cardiopulmonary resuscitation

作　　者：陈娣[1] 潘昊[1] 李醇文[2] 兰秀彩[1] 刘蓓蓓[1] 杨光田[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,武汉430030 [2]重庆医科大学附属第二医院急救部

出　　处：《中华急诊医学杂志》2011年第7期687-691,共5页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金资助项目（81071526）

摘　　要：目的研究心肺复苏后大鼠心肌细胞缺氧诱导因子-α（hypoxia-induciblefactor-α，HIF-α）的变化及外源性硫化氢（hydrogen sulfide，H2S）的干预作用。方法实验在华中科技大学同济医学院附属同济医院动物实验室进行，雄性SD大鼠45只，采用窒息法建立大鼠心搏骤停模型，随机（随机数字法）分为3组，每组15只：（1）对照组，仅行麻醉、气管插管及动静脉插管操作；（2）CPR组，进行模型制备及复苏；（3）CPR＋硫氢化钠（NaHS）组，以NaHS作为H2S供体，在CPR开始前1min经股静脉给予50μg/kg NaHS，其余操作同CPR组。观察各组血流动力学参数，留取6h心肌组织，采用RT-PCR法检测HIF-1α mRNA表达，采用MPO测定试剂盒测定各组心肌组织MPO含量，三组动物于24h处死取心肌组织行病理学检查。采用单因素方差分析，组间比较采用t检验。结果CPR组和CPR＋NaHS组的血流动力学指标差异无统计学意义（P〉0．05）。与对照组相比，CPR组和CPR＋NariS组HIF-1α基因表达均上升，CPR＋NaHS组上升更为显著（P〈0．05），与CPR组比较，CPR＋NariS组HIF-1α基因表达升高（P〈0．05）；与对照组相比，CPR组和CPR＋NariS组心肌MPO活性出现不同程度升高（P〈0．05），与CPR组比较，CPR＋NariS组心肌MPO活性下降（P〈0．05）；通过对心肌苏木精-伊红（HE）染色切片观察显示，CPR组和CPR＋NariS组出现不同程度的病理损伤，CPR＋NariS组的病理损伤轻于CPR组。结论HIF-1α mRNA在心肺复苏后大鼠心肌细胞的表达增强，硫化氢可能通过上调HIF-lα的表达，对心肺复苏后心肌起保护作用。Objective To investigate the changes of the expression of HIF-lα （hypoxia inducible factor-l α） mRNA in myocardium of rats ＇after cardiopulmonary resuscitation （CPR） and the intervention effects of exogenous hydrogen sulfide on it. Method Forty five male SD rats were randomly divided into three groups ： control group （ n = 15 ） , cardiopulmonary resuscitation group （ CPR group, n = 15 ） and Naris ＋ CPR group （ n = 15 ） . Rats of control group were anesthetized and intubated without asphyxia and cardiac arrest. The rats of CPR group and Naris ＋ CPR group were operated to induce cardiac arrest by asphyxiation. In the rats of NariS ＋ CPR group, Naris in dose of 50 ug/kg was administrated via the femoral venous line 1 minute before CPR. Hemodynamic was mooitored continuously. The expression of HIF - 1 α mRNA in myocardium of rats in each group was determined by using RT-PCR and the activity of myeloperoxidase （MPO） in the myocardium of rats in each group was assayed by using a patent reagent box 6 h after CPR. The histopathological changes of myocardium were also observed. The t- test was used for statistical analysis. Results There was no statistically significant difference in hemodynamic changes between CPR group and CPR ＋ Naris group （P 〉 0. 05 ） . When compared with the control group, the activity of MPO and the expression of HIF-lc~ mRNA in CPR group and CPR ＋ NariS group were both increased, and those increased in CPR ＋ NariS group was more significant （P 〈0. 05） . When compared with CPR group, the expression of HIF-lc~ mRNA in CPR ＋ NariS group was higher, however, the activity of MPO in CPR ＋ NariS group was lower （ P 〈 0. 05 ） . There were various histopathological changes of myoeardium of rats found in CPR group and CPR ＋ NariS group, and the damage of myocardium of rats it, CPR group was more obvious than that in CPR ＋ Naris group. Conclusions The expression of HIF-Iα mRNA in myocardium of rats was increased after CPR. Exogenous hydroge

关 键 词：心肺复苏 心肌损伤 硫化氢 缺氧诱导因子1-Α 髓过氧化物酶 

分 类 号：R459.7[医药卫生—急诊医学]