氢气饱和生理盐水对百草枯中毒大鼠肺组织的作用  

Effects of hydrogen saturated saline on the lung after paraquat poisoning

作　　者：张慧利[1] 柳远飞[1] 罗序睿[1] 谭位华[1] 陈耿靖[1] 

机构地区：[1]南昌大学第一附属医院急诊科,南昌330006

出　　处：《中华急诊医学杂志》2011年第7期708-711,共4页Chinese Journal of Emergency Medicine

摘　　要：目的探讨氢气饱和生理盐水对百草枯（PQ）中毒大鼠肺损伤及肺纤维化的保护作用。方法该实验在南昌大学第一附属医院动物实验室完成。48只SD大鼠随机分为对照组、染毒组和干预组，每组16只。染毒组及干预组给予50mg／kgPQ一次性灌胃染毒，对照组给予等量蒸馏水灌胃。干预组大鼠在染毒1h后开始予腹腔注射氢气饱和生理盐水5mL／kg，2次／d，直至处死；染毒组及对照组大鼠按体质量给予等量生理盐水腹腔内注射。于染毒第3天及第21天分别测定各组大鼠动脉血氧分压（PaO2）、肺组织8-羟基脱氧鸟苷（8-OHDG）含量及转化生长因子βl（TGF-β1）的表达。结果（1）染毒后3d，与对照组比较，染毒组及干预组PaO2均明显下降（P〈0．01）；与染毒组比较，干预组PaO2有所改善（P〈0．05）。21d后，与对照组比较，染毒组PaO2仍明显减低（P〈0．01），干预组有一定程度下降（P〈0．05）；与染毒组比较，干预组PaO2明显升高（P〈0．01）。（2）染毒后3d，与对照组比较，染毒组8-OHDG含量明显增加（P〈0．01），干预组亦有一定程度增加（P〈0．05）；与染毒组比较，干预组8-OHDG含量有明显下降（P〈0．01）。21d后，各组间比较差异无统计学意义（P〉0．05）。（3）染毒后3d，与对照组比较，染毒组TGF-β1表达明显增加（P〈0．01），干预组有一定程度增加（P〈0．05）；干预组较染毒组间比有一定下降（P〈0．05）。21d后，与对照组比较，染毒组TGF-β1表达明显增加（P〈0．01），干预组有一定增加（P〈0．05）；与染毒组比较，干预组TGF-β1表达明显下降（P〈0．01）。结论氢气饱和生理盐水能改善PQ大鼠氧化应激状态，减轻肺氧化损伤，降低肺纤维化程度。Objective To investigate protective effects of the hydrogen saturated saline on acute lung injury and pulmonary fibrosis in rats with paraquat （PQ） poisoning. Method Forty-eight Sprague-Dawley rats were randomly divided into 3 groups, namely control group, PQ poisoning group and intervention group （ n = 16 rats in each group） , Animals in PQ poisoning group and intervention group were fed with PQ in dosage of 50 mg/ kg. Rats of control group were fed equivalent amount of distilled water instead. One hour after administration of PQ, rats of intervention group were treated with 5 ml / kg hydrogen saturated saline injected intra-peritoneally twice a day until the rats were sacrificed. The rats of poisoning group and control group were treated with intra-peritoneal injection of equivalent amount of normal saline. Arterial partial pressure of oxygen （PaO2）, 8-hydroxy-2＇ -desoxyguanosine （8-OHDG） and transforming growth factor 131 （TGF-β1） of lung tissue were measured on the 3rd and 21st day after PQ administration. Quantitative data was expressed as mean± standard deviation （x± s） . SPSS version 12. 0 package was applied for variance analysis and SNK-q test, and statistical differences were considered significant when P 〈 0. 05. Results ① PaO2 decreased significantly in poisoning group （9.34 ± 0. 47 kPa） and intervention group （10. 30 ± 0.62 kPa） compared with control group （11.87 ±0.42 kPa） on the 3d （P 〈0.01）, and as intervention group was compared with poisoning group, there was a significant difference （ P 〈 0. 05 ） . On the 21 st day, PaO2 was still lower in poisoning group （8. 36±0. 51 kPa） and intervention group （ 10. 14 ±0. 27 kPa） than that in control group （ 11.87±0. 24 kPa） （P 〈0. 01 and P 〈0. 05, respectively）, and as intervention group was compared with poisoning group, there was a significant difference （ P 〈 0. 01 ） .② The levels of 8-OHDG in lung tissue increased significantly in poisoning group （23.58±7. 18 n

关 键 词：百草枯 氧化应激损伤 肺 氢气饱和生理盐水 8-羟脱氧鸟苷 转化生长因子 β1 大鼠 

分 类 号：R595.4[医药卫生—内科学]