血小板内皮细胞黏附分子-1与百草枯中毒后兔肺损伤的关系  

The relationship between platelet endothelial cell adhesion molecule-1 （PECAM-1） and Paraquat induced lung injury in rabbits

作　　者：史晶[1] 胡春林[2] 高渝峰[1] 廖晓星[2] 

机构地区：[1]广州市第十二人民医院广州市化学中毒救援中心急诊科,广州510620 [2]中山大学附属第一医院急诊科

出　　处：《中华急诊医学杂志》2011年第7期712-716,共5页Chinese Journal of Emergency Medicine

基　　金：广东省医学科研基金项目（2010501）;广州市医药卫生科技项目（2009.YB-111）

摘　　要：目的探讨百草枯中毒后肺组织内血小板内皮细胞黏附分子-1（PECAM-1）表达的变化与肺损伤和肺纤维化程度的关系。方法36只成年新西兰大白兔，依据胃管内灌入百草枯剂量的不同，随机分为8mg／kg（A组）、16mg／kg（B组）和32mg／kg（C组）。中毒后观察动物生存状况；7d后处死动物，取肺组织进行组织学损伤评分、Masson染色鉴定肺纤维化（LF）程度、免疫组化半定量分析PECAM-1的表达，Pearson相关分析法确定PECAM-1表达和ALI、LF程度的关系。结果每组12只动物，均出现明显的中毒症状，C组存活时问为（6．47±0．99）d，短于B组的（6．09±1．04）d（P=0．031）和A组的（4．77±2．04）d（P=0．007）。ALI评分A组为（8．33±1．03），低于B组的（9．834-1．17）（P=0．047）和C组的（11．50±1．38）（P〈0．01），B组与C组相比较，P=0．03。肺纤维化程度A组为（31．09±2．05）％，低于B组的（34．37±1．62）％（P=0．002）和C组的（36．54±0．44）％（P〈0．01），B组和C组之间差异具有统计学意义（P=0．026）。A组肺PECAM-1的表达为（20．31±0．70）％，高于B组的（19．344-0．68）％（P=0．16）和C组的（18．37±0．46）％（P〈0．01），B组与C组相比，差异具有统计学意义（P=0．017）。Pearson相关分析显示，PEEAM-1的表达和ALI评分（Coe=-0．732，P=0．001）、肺纤维化程度（Coe=-0．779，P〈0．001）明显相关。结论新西兰兔PQ中毒后，肺组织内PECAM-1表达明显下降，成剂量依赖性，与肺损伤程度、肺纤维化程度密切相关；PECAM-1表达的下降在PQ致肺损伤的发生、发展中起重要作用。Objective To explore the relationships between the expression of PECAM-1 and the degree of ALl in Paraquat induced lung injury model of rabbits. Method Thirty six adult New Zealand rabbits were randomly divided into three groups： 8 mg/kg （Group A）, 16 mg/kg （Group B） and 32 rag/ kg （Group C） according to the dose of Paraquat which were infusion into stomach. After poisoned, the animals were monitored for seven days, and then sacrificed. The upper lobe of lung were removed for HE, Masson staining and immunohistochemisty. The ALI score, fibrosis of lung and expression of PECAM-1 were semiquantitative analyzed. Results Each group has 12 animals suffered from poisoning. The survival time of animals in Group C was （6. 47 ±0. 99 ） days, shorter than （6. 09 ±1.04） days （ P = 0. 031 ） in Group B and （4. 77 ±2. 04） days （P = 0. 0 07） in Group A. The ALI score were （8. 33 ± 1.03） points in Group A, superior to （9. 83 ±1.17） points （P = 0. 047） in Group B and （ 11.50 ± 1.38） points （P 〈 0. 01 ） in group C, Group B vs Group C, P = 0. 03 o The fibrosis degree of lung was （31.09 ± 2. 05） % in Group A, not severe as （34.37±1.62）% （P=0.002） in Group B and （36.5±0.44）% （P〈0.01） in Group C, Group B vs Group C, P = 0. 026. The Pearson correlation analysis showed the expression of PECAM-1 was negative correlated to ALl score （Coe = -0. 732, P = 0. 001 ） and fibrosis degree of lung （Coe = - 0. 779, P 〈 0. 001 ） . Conclusions The expressions of PECAM-1 were significantly decreased in New Zealand after Paraquat poisoned, which were dose dependent, correlated to ALI scored and fibrosis degree of lung, so it may play an important role in the development of lung injured induced by Paraquat.

关 键 词：百草枯 急性中毒 血小板内皮细胞黏附分子-1 急性肺损伤 

分 类 号：R595.4[医药卫生—内科学]