重楼醇提取物含药血清对胃癌SGC7901细胞增殖和凋亡影响的观察  

Effects of rhizoma paridis pharmacologic serum on proliferation and apoptosis of human gastric carcinoma cell line SGC7901 in vitro

作　　者：张珂[1,2] 牟翰舟[3] 冯建国[3] 顾琳慧[3] 马胜林[1,2] 

机构地区：[1]杭州市第一人民医院 [2]杭州市肿瘤医院肿瘤科,浙江杭州310006 [3]浙江省肿瘤医院肿瘤研究所,浙江杭州310002

出　　处：《中华肿瘤防治杂志》2011年第12期932-935,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：浙江省中医药重点研究项目(2011ZZ011)

摘　　要：目的:观察重楼醇提取物含药血清对胃癌SGC7901细胞增殖和凋亡的影响。方法:以重楼醇提取物不同浓度含药血清培养SGC7901细胞后,以MTT法检测对细胞增殖的影响,流式细胞仪检测细胞周期和细胞凋亡变化。结果:不同给药剂量组含药血清作用于SGC7901细胞24、48和72h后,细胞生存率显著降低,且与时间和剂量呈依赖关系。不同给药剂量组含药血清作用于SGC7901细胞24h后,细胞阻滞发生在S期;凋亡率分别为(6.67±1.34)%、(13.12±1.34)%和(20.43±1.24)%,联合用药组为(22.61±3.01)%,空白组动物血清组凋亡率(4.35±1.09)%及氟尿嘧啶(5-FU)组(19.22±1.66)%比较,差异均有统计学意义,P<0.01。结论:重楼醇提取物含药血清对SGC7901细胞生长有明显抑制作用,并可诱导SGC7901细胞凋亡。OBJECTIVE： To explore the effect of rhizoma paridis pharmacologic serum on proliferation and apoptosis of human gastric carcinoma cell line SGC7901 in vitro. METHODS： SGC7901 cell line was cultured with different concentration of rhizoma paridis pharmacologic serum, and its proliferation and apoptosis was detected by MMT and flowcytometric, repectively. RESULTS： The survival of SGC7901 cells was decreased after 24, 48, 72 h after culturing with different concentration of rhizoma paridis pharmacologic serum, and it was correlated with the cultu- ring time and contents. After 24 h culturing with rhizoma paridis pharmacologic serum, the cells in S stage were increased; the apoptosis rate of the 3 groups was （6.67±1.34）%, （13.12 ± 1.34）%, （20.43± 1.24）%, and 5-FU add Rhizoma Paridis was （22.61 ± 3.01）% as compared with control group （4.35 ± 1.09）% and fluorouracil group （ 19.22 ± 1.66）%, respectively, with a significant difference （P〈0.01）. CONCLUSION ： Rhizoma paridis pharmacologic serum can inhibit the growth of SGC7901, and may induce the apoptosis of SGC7901 cells.

关 键 词：胃肿瘤 重楼含药血清 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]