光学蛋白芯片法定量检测血清CA15-3抗原方法学研究  

Detection of CA15-3 antigen in serum using optical protein chip based on imaging ellipsometry biosensor

作　　者：向双[1] 牛宇[2] 孟永宏[2] 靳刚[2] 王元书[1] 赵子彦[1] 

机构地区：[1]山东省医学科学院药物所山东省罕少见病重点实验室,山东济南250062 [2]中国科学院力学研究所,北京100080

出　　处：《中华肿瘤防治杂志》2011年第11期821-824,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家863项目(2008AA02Z419)

摘　　要：目的:建立以椭偏光生物传感器为基础的光学蛋白芯片(OPC-IE)定量检测CA15-3抗原方法。方法:探索OPC-IE生物分子芯片系统检测CA15-3抗原方法及建立了CA15-3抗原浓度-灰度检测标准曲线,依据标准曲线定量检测了80份血清(对照组及癌症患者组)样本,并将检测结果与电化学发光法(ECLIA)进行比较。结果:所建立的OPC-IE检测CA15-3方法,在46.33、23.16和11.58 IU/mL的组间变异系数(n=6)分别为7.67%、10.57%和9.40%,试验结果有良好重复性。浓度-灰度标准曲线的R2=0.97,检测最小值为5.30 IU/mL。对照组CA15-3抗原浓度为(2.84±0.73)IU/mL,癌症患者组为(52.45±7.50)IU/mL,两组浓度差异有统计学意义,P<0.05。ROC曲线和t检验分析80份血清OPC-IE和ECLIA检测结果,显示差异无统计学意义,P>0.05。结论:初步建立了OPC-IE CA15-3抗原检测方法,并且成功的应用于血清检测。OBJECTIVE：To investigate the feasibility of optical proteinchip based on imaging ellipsometry biosensor （OPC-IE） applying to CA15-3 antigen assay. METHODS： The technological method for detecting CA15-3 antigen by OPC-IE and its concentration-gray standard curve were established, then 80 serum samples from both normal and cancer patients were ex- amined. The detection results of CA15-3 antigen were compared with detections by electrochemistry luminescence method （ECLIA）. RESULTS Our method for detecting CA15-3 antigen showed a good repeatability, the coefficient of variation values were 7.67%, 10.57% and 9.40% at 46.33, 23.16 and 11.58 IU/mL, respectively （n= 6）. Pearson correlation coeffi cient （R2） of concentration-gray scale standard curve was 0.97, and the lowest detectable limit of standard curve was 5.30 IU/mL. Concentrations of CA15-3 antigen were differed significantly in normal （2.84±0.73） IU/mL and cancer patients （52.45±7.50） IU/mL （P〈0.05）, There was no significant difference between the detections by OPC-IE and ECLIA （P〉 0.05）. CONCLUSION： A method for detecting CA15-3 antigen was established and it was tested in clinical serum examination.

关 键 词：蛋白质阵列分析 抗原 CA15-3/血液 肿瘤标记 生物学 

分 类 号：R446.6[医药卫生—诊断学]