黑种草籽调节黑素合成的药效物质基础  

Pharmacodyamic Material Basis of Nigella sativa L. Regulating Melanogenesis

作　　者：范雪琦[1] 徐建国 廖莎[1] 周佳[1] 杨伟峻 尚靖[1] 

机构地区：[1]中国药科大学新药筛选中心,南京210009 [2]新疆维吾尔自治区卫生厅,乌鲁木齐830001 [3]新疆维吾尔自治区药物研究所,乌鲁木齐830004

出　　处：《吉林大学学报（理学版）》2011年第4期787-791,共5页Journal of Jilin University:Science Edition

基　　金：国家科技支撑项目(批准号:2007BAI30B03);教育部"新世纪优秀人才支持计划"项目(批准号:NCET-07-0851);国家"十一五"科技重大专项基金(批准号:2009ZX09302-002)

摘　　要：以高效液相色谱(HPLC)法对黑种草籽不同体积分数的醇提取物进行色谱峰表征,以体外培养的小鼠B16黑素瘤细胞为模型,采用MTT法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞增殖的影响,采用L-DOPA氧化法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞酪氨酸活性的影响,采用NaOH裂解法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞黑色素含量的影响,并结合药理研究结果对不同体积分数醇提取物的HPLC图谱进行相关性统计分析.实验结果表明:黑种草籽体积分数为50%的乙醇提取物具有促进小鼠黑素瘤细胞B16增殖及促进黑素合成的作用;谱效分析HPLC图谱中的6个色谱峰对药效的贡献度无差异,表明黑种草籽药效是中药多组分协同作用.Chromatographic peaks of different concentration of Nigella sativa L.extacts were characterized by HPLC.B16 murine melanoma cells were used as an in vitro cultured model.MTT was used to study the effect of Nigella sativa L.ethanolic extracts on the proliferation of B16 murine melanoma cells.Oxidation rate of L-DOPA was measured to estimate the effect of Nigella sativa L.ethanolic extracts on tyrosinase activity of B16 murine melanoma cells.NaOH cleavage method was performed to study the effect of Nigella sativa L.ethanolic extracts on the melanogenesis of B16 murine melanoma cells.Statistical analysis to the chromatographic peaks of different concentration of Nigella sativa L.extacts and pharmacodynamic data for correlation analysis.Results show that 50% ethanolic extract of Nigella sativa L.enhances melanogenesis and growing rate of B16 murine melanoma cells.Component-effect relationship shows that there is no significant difference in the 6 chromatographic peaks on pharmacodynamic effect.Pharmacodynamic effect is due to the multiple-comonents of Nigella sativa L.

关 键 词：黑种草籽 细胞增殖 酪氨酸酶活 黑素合成 相关分析 

分 类 号：R965.1[医药卫生—药理学]