苦碟子总黄酮对脑缺血性损伤保护作用的实验研究  

Protective effects studing of total flavonoids of sowthistle-leafixeris seedling on cerebral ischemia injury in rats

作　　者：贾德武[1] 罗胜勇[2] 叶寿山 贾德云 李心伟[2] 马征[2] 李睿[2] 

机构地区：[1]安徽医科大学附属医院药剂科 [2]安徽省医学科学研究院

出　　处：《中国临床药理学与治疗学》2011年第6期616-620,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究苦碟子总黄酮(TFS)对小鼠急性脑缺血和大鼠局灶性脑缺血的保护作用。方法:结扎小鼠双侧颈总动脉建立脑缺血模型,观察TFS对小鼠死亡率及死亡时间的影响。建立局灶性脑缺血(MCAO)模型,观察TFS对大鼠行为学、脑梗死范围的影响。测定脑含水量及脑组织中一氧化氮合酶(NOS)和诱导型一氧化氮合酶(iNOS)含量。结果:与模型组比较,TFS16、8 mg/kg组可降低缺血小鼠的死亡率,延长死亡时间(P<0.05,P<0.01);16、8、4 mg/kg组可改善行为障碍,降低行为学评分(P<0.05,P<0.01);105、mg/kg组可减少MCAO大鼠脑梗死范围,降低脑组织含水量及NOS和iNOS含量(P<0.05)。结论:TFS对缺血性脑损伤有保护作用,其机制可能与抑制iNOS有关。AIM：To investigate the protective effects of total flavonoids of sowthistle-leafixeris seedling（TFS） against acute cerebral ischemia in mice and focal cerebral ischemia in rats. METHODS： The occlusion of bilateral common carotid arteries with vagus in mice was used for make the acute cerebral ischemia models.The survival time and the death rate were observed.The permanent occlusion of the proximal of the right middle cerebral artery（MCA）was used for make the focal cerebral ischemia models.The extent of neurological deficits was observed,and the infarct area was measured by NBT staining technique,investigating the influence of TFS on the contents of NOS and iNOS.RESULTS：Compared with model group,TFS of 16,8 mg/kg prolonged the survival time and decreased the death rate of mice with acute cerebral ischemia injury（P0.05,P0.01）.TFS of 16,8,4 mg/kg ameliorated neurologic deficits score and the infarct size of rats with MCAO,reduced the contents of NOS,iNOS（P0.05）.CONCLUSION： TFS has profective effects on cerebral ischemia injury,the mechanism may relate to the inhibiton of NOS and iNOS.

关 键 词：苦碟子总黄酮 脑缺血 一氧化氮合酶 诱导型一氧化氮合酶 

分 类 号：R965.2[医药卫生—药理学]