光动力疗法对增生性瘢痕成纤维细胞增殖影响的研究  

Influence of ALA-PDT on Fibroblast Cells from Hypertrophic Scars

作　　者：钟俊波[1] 王亮[1] 罗利达[1] 潘玲[1] 

机构地区：[1]自贡市第四人民医院烧伤整形科,自贡643000

出　　处：《四川大学学报（医学版）》2011年第4期515-518,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川省卫生厅科研基金(项目编号090571)资助

摘　　要：目的探讨以5-氨基酮戊酸(ALA)为光敏剂的光动力疗法(PDT)在体外对增生性瘢痕成纤维细胞(FB)增殖的影响,并探寻合适的光敏剂浓度和激光能量密度。方法组织块法体外培养人增生性瘢痕FB。实验分为空白对照组、激光组(仅予不同能量密度激光照射)、光敏剂组(仅予不同浓度光敏剂同时培养)和ALA-PDT组(光敏剂培养+激光照射)。用CCK-8检测各组细胞增殖抑制率,根据结果选出最佳的ALA浓度和激光能量密度的组合,检测该组合干预后的FB增殖细胞核抗原(PCNA)的表达水平。结果激光组和光敏剂组对增生性瘢痕FB增殖无明显抑制作用。ALA-PDT组中,当激光剂量≥10 J/cm2与药物浓度≥0.125 mmol/L时,抑制作用明显,随激光能量和药物浓度的同时增大,抑制作用逐渐增大。而当ALA浓度达到0.5 mmol/L,激光能量密度达到20 J/cm2后,再增大药物浓度和激光能量抑制率无明显升高,在此条件下的ALA-PDT组PCNA表达水平低于空白对照组(30.33±2.08 vs.78.33±3.79,P<0.05)。结论 ALA-PDT对体外培养的人增生性瘢痕成纤维细胞增殖有明显抑制作用,其抑制作用随药物浓度和激光能量密度增加而增加。最佳参数:ALA浓度为0.5 mmol/L,He-Ne激光能量密度为20 J/cm2。Objective To determine the effect of ALA-PDT on fibroblast cells from hypertrophic scars in vitro,and to identify proper dosage of photosensitizer and energy level of laser light.Methods Fibroblast cells from hypertrophic scars were cultured in vitro.ALA（5-aminolevulnilic acid） was added into the culture fluid with different concentrations.Then the cultured cells were exposed to He-Ne laser light（PDT） with different energy levels.The cell inhibition rate was measured with CCK-8 method.The expression of PCNA in fibroblast cells was detected after cultured with ALA-PDT,which was selected according to the proper dosage of ALA and laser light from the result of CCK-8.Results No obvious inhibition effect was detected with laser light or photosensitizer alone on fibroblast cells from hypertrophic scars.The inhibition effect appeared when ALA and PDT applied simultaneously,and the inhibition rate increased with the dosage of photosensitizer and the energy level of laser light.Maximal inhibition effect reached with 20 J/cm2 laser light exposure and 0.5 mmol/L of ALA,the expression of PCNA of ALA-PDT group is lower than control group in this condition（30.33±2.08 vs.78.33±3.79,P0.05）.Conclusion The inhibition effect of ALA-PDT on fibroblast cells from hypertrophic scars increases with the dosage of photosensitizer and the energy level of He-Ne laser light.The proper dosage of ALA is 0.5 mmol/L along with 20 J/cm2 laser light exposure.

关 键 词：5-氨基酮戊酸 光动力疗法 增生性瘢痕 成纤维细胞 

分 类 号：R622[医药卫生—整形外科]