相似蜂海绵相关真菌杂色曲霉F62活性代谢产物研究  

The bioactive metabolites of Aspergillus versicolor F62 isolated from Haliclona simulans

作　　者：董世豪[1] 巩婷[1] 朱平[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所卫生部天然药物生物合成重点实验室中草药物质基础与资源利用教育部重点实验室,北京100050

出　　处：《菌物学报》2011年第4期636-643,共8页Mycosystema

基　　金：国家"十一五"科技重大专项"综合性新药研究开发技术大平台"(No.2009ZX09301-003-4-1)

摘　　要：研究了1株相似蜂海绵相关真菌F62的活性代谢产物,经分子系统学分析表明该真菌属于杂色曲霉。将F62菌株用大米固体培养基在室温(约25℃)下静置培养45d,经乙酸乙酯超声萃取得到粗提物。通过硅胶柱层析、Sephadex LH-20凝胶柱层析和HPLC等色谱手段,分离得到了6个化合物。通过核磁共振、质谱等波谱分析手段可鉴定出其结构分别为Alantrypinone(1)、洛伐他汀(2)、甲酯型莫纳克林K(3)、土曲霉酮(4)、土震素B(5)和麦角甾醇(6)。化合物1系首次从该属真菌中分离得到,化合物2-5为首次从该种真菌中分离得到。首次对化合物3的碳谱数据进行报道。初步的药理研究表明,化合物4具有体外抗炎活性。A marine fungal strain F62 primarily determined as Aspergillus versicolor was isolated from sponge Haliclona simulans.The sponge-associated fungus was cultivated on the rice solid medium at room temperature （about 25℃） for 45 days.The culture was extracted with ethylacetate and the total extract was purified by means of silica gel,Sephadex LH-20 column chromatography,high performance liquid chromatography （HPLC）,and so on.Six compounds were obtained and the structures were determined as alantrypinone （1）,lovastatin （2）,methylester of lactone ring-opened monacolin K （3）,terrein （4）,territrems B （5） and ergosterol （6）,respectively,based on nuclear magnetic resonance （NMR）,mass spectrum （MS） and other spectroscopic determination.Compound 1 and compounds 2-5 were first isolated from the genus Aspergillus and Aspergillus versicolor,respectively.13C NMR spectral data of compound 3 were provided for the first time.Compound 4 was first reported to have anti-inflammatory activity in vitro.

关 键 词：海绵相关真菌 杂色曲霉 次生代谢产物 活性 

分 类 号：Q946[生物学—植物学]