肝再生磷酸酶-3小干扰RNA对异位子宫内膜间质细胞迁移的影响  

Effects of PRL-3 siRNA on the migration of endometriotic stromal cells RUAN

作　　者：阮菲[1] 吴瑞瑾[1] 徐开红[1] 马俊彦[1] 鲁东红[1] 陈利青[1] 涂飞霞[1] 梁峥[1] 林俊[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,杭州310006

出　　处：《中华医学杂志》2011年第23期1613-1616,共4页National Medical Journal of China

基　　金：国家自然科学基金（30973171）;浙江省卫生厅科学基金（2009A129）

摘　　要：目的 通过siRNA体外沉默肝再生磷酸酶-3（PRL-3）,探讨其对异位子宫内膜间质细胞迁移的影响.方法 原代培养异位子宫内膜间质细胞,运用siRNA技术干扰沉默PRL-3基因,Western blot检测干扰后PRL-3蛋白表达变化,观察细胞板状伪足的形成,采用划痕实验、transwell细胞迁移实验观察并比较细胞迁移能力的改变.结果 经siRNA 干扰沉默后实验组PRL-3蛋白相对表达量较对照组明显下降（P〈0.05）,细胞形成的板状伪足与对照组相比明显减少,相同时间内发生定向迁移的实验组细胞数为0.87±0.21,空白对照组为1.75±0.28,阴性对照组为1.63±0.39,前者与后两者比较差异有统计学意义（P〈0.05）.结论 PRL-3 siRNA能有效沉默PRL-3基因,并且使异位子宫内膜间质细胞的迁移能力下降.Objective To evaluate the effects of PRL-3 siRNA （small interfering RNA） on the migration of endometriotic stromal cells.Methods Primary endometriotic stromal cells were cultured in vitro.Then PRL-3 （phosphatase of regenerating liver-3） siRNA was transfected to silence the PRL-3 gene.And the PRL-3 protein expression was analyzed by Western blot.The changes of cell migration were detected by cell pseudopod formation, scratch test and transwell cell migration test.Results The expression of PRL-3 protein significantly decreased in the experimental group versus two other control groups （P〈0.05）.The formation of cell pseudopod was much less in experimental group than that in control groups. Within the same time, the number of migration cells was 0.87±0.21 in experimental group.It was less than 1.75±0.28 in blank control group and 1.63±0.39 in negative control group（P〈0.05）.Conclusion PRL-3 siRNA can down-regulate the PRL-3 gene and decrease the migratory capacity of endometriotic stromal cells.And PRL-3 may be a promising target in the therapeutics of endometriosis.

关 键 词：子宫内膜异位症 肝再生磷酸酶-3 小干扰RNA 迁移 

分 类 号：R711.71[医药卫生—妇产科学]