兔下颌骨前牙区剩余牙槽嵴模型的建立  

Establishment of a rabbit model of anterior residual alveolar ridge

作　　者：胡杨[1] 马莹 何惠宇[1] 

机构地区：[1]新疆医科大学第一附属医院口腔修复科,新疆维吾尔自治区乌鲁木齐市830054 [2]广州市天河区中心医院口腔科,广东省广州市510630

出　　处：《中国组织工程研究与临床康复》2011年第20期3653-3656,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：新疆维吾尔自治区科技厅科技攻关项目(200533118);课题名称:组织工程骨用于即刻牙种植的骨结合研究;新疆维吾尔自治区高校科研计划科学研究重点项目(xj EDU2009I22);课题名称:bFGF基因转染的骨髓间充质干细胞复合异种煅烧骨修复颌骨缺损的实验研究;乌鲁木齐市科学技术计划项目(Y09131002);课题名称:牙周植骨术联合固定义齿夹板保留牙周炎松动牙的临床应用研究~~

摘　　要：背景:建立合适的牙槽嵴缺损动物模型可为开展剩余牙槽嵴的保存、修复研究奠定基础。目的:建立兔下颌骨前牙区剩余牙槽嵴实验动物模型。方法:全麻下完整拔除18只新西兰大白兔双侧下颌中切牙建立兔下颌骨前牙区剩余牙槽嵴模型。术后4,8,12周,处死白兔,游标卡尺测量兔下颌剩余牙槽嵴的长度、宽度和高度,苏木精-伊红染色观察拔牙窝的愈合程度,X射线摄片观察兔下颌拔牙创内骨小梁改建情况。结果与结论:随着手术时间的延长,牙槽嵴长度、宽度和高度均逐渐降低;拔牙窝内有成骨细胞散在分布,新生骨小梁和血管网逐渐形成,牙槽窝持续沉积新生骨组织,与周围牙槽骨界限不清,骨改建生成,拔牙创愈合。苏木精-伊红染色及X射线片均证实兔前牙区剩余牙槽嵴模型建立成功。BACKGROUND：A proper anterior residual alveolar ridge model can provide basis for the preservation and repair of residual alveolar ridge.OBJECTIVE：To establish a rabbit animal model of anterior residual alveolar ridge.METHODS：The eighteen New Zealand white rabbits were selected,both side of central incisors of 18 rabbits were extracted under the general anesthesia to prepare anterior residual alveolar ridge models.Rabbits were killed at 4,8,and 12 weeks after operation.The length,width and height of residual alveolar after extraction were measured by Vernier caliper.The healing process of teeth extraction was observed by hematoxylin-eosin staining.The remodeling of bone trabecula in after extraction was examined by X-ray.RESULTS AND CONCLUSION：The length,width and height of residual alveolar ridge were gradually decreased with time prolonged.Osteoblasts could be seen scattered in teeth extraction,new trabecular bone and vascular network was born.The deposition of new bone was continued in teeth extraction,the boundaries between new bone and the alveolar bone was not clear,and new bone and bone remodeling were happening in teeth extraction.Hematoxylin-eosin staining and X-ray photo showed that rabbit models of anterior residual alveolar ridge were successfully established.

关 键 词：剩余牙槽嵴 下颌骨前牙区 动物模型 兔 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]