β-磷酸三钙/聚-L-乳酸复合材料的骨内降解  

In vivo degradation of beta-tricalcium phosphate/poly-l-lactide composites in cancellous bone

作　　者：王晓[1,2] 刘玉杰[1] 陈旭[1] 

机构地区：[1]解放军总医院骨科六区,北京市100853 [2]解放军第62医院骨科,云南省普洱市665000

出　　处：《中国组织工程研究与临床康复》2011年第21期3807-3810,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:调控聚乳酸类可吸收材料的降解速率,使材料降解与新生骨爬行替代速度更同步,加入羟基磷灰石或β-磷酸三钙等无机粒子是目前的主流选择。目的:对比观察β-磷酸三钙/聚-L-乳酸复合材料和聚-L-乳酸在松质骨内的降解速度及诱导成骨能力。方法:将β-磷酸三钙/聚-L-乳酸复合材料及聚-L-乳酸材料分别植入12只新西兰大白兔双侧股骨内髁及外髁后,于术后6,12,24周3个时间点取材,测定其各个时间点的生物吸收率,扫描电镜和光学显微镜观察其在松质骨内的形态学变化,周围新骨爬行替代及异物反应情况。结果与结论:各个时间点β-磷酸三钙/聚-L-乳酸与周围骨质贴合比聚-L-乳酸材料更紧密,未见明显异物反应。6周时β-磷酸三钙/聚-L-乳酸材料生物吸收率小于聚-L-乳酸材料,12周后生物吸收率增速加快,同时材料表面出现均匀分布的微孔及裂隙;术后24周内两种材料均未见新生骨爬行替代。结果表明β-磷酸三钙/聚-L-乳酸复合材料早期降解较聚-L-乳酸材料慢,有利于移植物植入早期的坚强固定;6周后降解加快,24周内未见诱导成骨现象。BACKGROUND：To improve the degradation and osteoinductive property of Poly-L-lactide（PLLA） materials,adding inorganic particles such as hydroxyapatite（HA）and β-tricalcium phosphate（β-TCP）is a popular choice.OBJECTIVE：To investigate the in vivo degradation and osteoinductive property of β-TCP/PLLA and PLLA materials in cancellous bone comparatively.METHODS：Totally 12 adult New Zealand rabbits were implanted PLLA andβ-TCP/PLLA materials into medial and lateral femoral condyle on both sides respectively.The rabbits were sacrificed separately at 6,12,24 weeks after operation,4 animals at each time.The bioabsorption rate,light microscopic and scanning electron microscopic images of PLLA and β-TCP/PLLA materials and surrounding bony tissues were investigated.RESULTS AND CONCLUSION：β-TCP/PLLA composites contacted more closely with surrounding bone tissues than PLLA.No foreign body reaction was observed.The bioabsorption rate of β-TCP/PLLA that was slower than PLLA in the first 6 weeks accelerated after this period,while more micropores and fine groove in the surfaces and inner part of β-TCP/PLLA composites were observed.No bony replacement was observed in 24 weeks postoperatively.The results show that the degradation rate of β-TCP /PLLA composites that are slower than PLLA in early stage speed up after this period,which is propitious to stablize fixation of implants.Tissue compatibility of β-TCP /PLLA composites is better than PLLA.However,no osteoinductive property of both materials is observed during the study periods.

关 键 词：聚-L-乳酸 β-磷酸三钙/聚-L-乳酸 生物可吸收材料 体内降解 诱导成骨 

分 类 号：R318[医药卫生—生物医学工程]