脱细胞枪乌贼神经的制备及其生物相容性  

Preparation and biocompatibility of new heterogeneous acellular nerve of squid

作　　者：范伟杰[1] 丁志清 徐雪梅[3] 

机构地区：[1]南华大学附属第一医院骨科,湖南省衡阳市421001 [2]株洲市第一人民医院骨科,湖南省株洲市412000 [3]南华大学图书馆,湖南省衡阳市421001

出　　处：《中国组织工程研究与临床康复》2011年第21期3843-3846,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖南省衡阳市科委资金资助项目(2007KJ006)资助;项目名称:脱细胞同种异体神经的优化与组织工程神经的保存研究~~

摘　　要：背景:脱细胞哺乳动物神经有有髓纤维直径小、细胞成分不易彻底清除、复合细胞不均匀的缺点。目的:制备脱细胞枪乌贼神经,并探讨其生物相容性。方法:用Hasse化学萃取法制备脱细胞枪乌贼神经,观察其脱细胞的效果、管道及基底膜的完整性,用MTT法检测脱细胞枪乌贼神经与细胞相容性,用皮下植入实验检测其组织相容性。结果与结论:制备的脱细胞枪乌贼神经细胞及髓鞘清除彻底、神经纤维膜管及基底膜保留完整,且膜管粗大。脱细胞枪乌贼神经皮下植入植入后1和2周材料周围有炎症细胞浸润。植入后4周材料周围有薄层纤维结缔组织囊,材料内部及周围只有少量炎症细胞。结果证实,采用Hasse化学萃取法制备的脱细胞枪乌贼神经,细胞清除彻底,神经纤维膜管及基底膜保留完整,与异种细胞及组织相容性良好。BACKGROUND：Acellular nerves of mammals have disadvantages：①The diameter of myelinated fiber is small;②The cellular components are difficult to eliminate;③The cells are uneven when the acellular nerves are made into tissue engineering nerve.OBJECTIVE：To prepare a new kind of squid acellular nerve and to explore its biocompatibility.METHODS：The acellular nerves of squid were prepared by chemical methods（Haase chemical extraction）.The effects of cells removed were estimated.The integrity of the nerve fiber tube and Schwann cell basilar membrane was observed.The compatibilities of acellular nerves of squid and cells were detected by MTT assay.Its histocompatibility was observed by subcutaneous implantation experiment.RESULTS AND CONCLUSION：The cells and myelin of acellular squid nerve prepared by Haase chemical extraction had been cleared completely,and the integrity of the nerve fiber tube and Schwann cell basilar membrane had been remained,and the tube of nerve fiber were large.Subcutaneous implantation results indicated that inflammatory cells expressed around the material at1 week,2weeks after implantation,and thin fibrous connective tissue capsule appeared around the material at 4 eeks after implantation,and few inflammatory cells expressed.The compatibility of xenogeneic cell and the compatibility of xenogeneic tissue were good.

关 键 词：脱细胞神经 枪乌贼 异种 组织工程 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]