改良自乳化溶剂扩散法制备MePEG-PLA纳米粒对成骨细胞的毒性  

Cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on osteoblasts in vitro

作　　者：王忠磊[1] 高岩[1] 赖春花[1] 卢海宾[1] 李少冰[1] 蒋颖[1] 韦丛云[1] 周磊[1] 

机构地区：[1]南方医科大学附属口腔医院广东省口腔医院,广东省广州市510280

出　　处：《中国组织工程研究与临床康复》2011年第21期3851-3854,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家"十一五"科技支撑计划(2007BAI18BO6)"牙列缺损及缺失的种植修复与功能重建的研究"~~

摘　　要：背景:两亲性嵌段聚合物由于其较强的载药能力强、纳米级大小、血液中长循环等优点在载药系统中得到广泛的应用。目的:评估改良自乳化溶剂扩散法制备的甲氧基封端的聚乙二醇-聚乳酸(MePEG-PLA)纳米粒对人骨肉瘤细胞MG63的毒性。方法:通过改良自乳化溶剂扩散法制备MePEG-PLA纳米粒,MTS法测定纳米粒培养1,2,3d后对MG63的毒性。激光粒度分析仪测定纳米颗粒的粒径大小、粒径分布及Zeta电位;透射电镜表征纳米胶束外观形态;酶标仪检测培养1,2,3d细胞吸光度值。结果与结论:MePEG-PLA纳米粒的平均粒径为25.7nm,分布均匀,呈球形,Zeta电位为-8.06mV,MePEG-PLA毒性为0级。提示改良自乳化溶剂扩散法制备纳米粒简单易行,制备的纳米粒无毒,具有良好的应用前景。BACKGROUND：Amphiphilic block copolymers have been widely applied in the drug delivery system because of the high capacity of drug carriers,nanoparticles and long circulation time.OBJECTIVE：To evaluate the cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on MG63 in vitro.METHODS：MePEG-PLA nanoparticles were prepared by modified self-emulsion solvent diffusion technique.The cytotoxicity effect of MePEG-PLA nanoparticles on MG63 was detected using MTS assay at days 1,2,3.Particles size,polydispersity index and Zeta electric potential were measured by laser particle size analzer.The morphology of MePEG-PLA nanoparticles were observed by transmission electron microscope.Absorbance values at days 1,2,3 were detected by microplate reader.RESULTS AND CONCLUSION：MePEG-PLA nanoparticles had a regular spherical shape with the mean diameter of 25.7 nm and scattered evenly.The Zeta potential was-8.06 mV.The cytotoxicity grade of MePEG-PLA nanoparticles was 0.The results indicate that MePEG-PLA nanoparticles prepared easily by modified self-emulsion solvent diffusion technique are nontoxic to the MG63 and have a good prospect.

关 键 词：纳米粒 药物载体 聚乳酸 聚乙二醇 改良自乳化溶剂扩散法 

分 类 号：R318[医药卫生—生物医学工程]