High efficient mixed culture screening and selected microbial community shift for bioleaching process  

高效浸矿混合菌种的筛选及其在浸矿过程中的微生物群落结构演变(英文)

作　　者：李寿朋[1,2] 郭宁[1,2] 武海艳[1,2] 邱冠周[1,2] 刘新星[1,2] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083 [2]中南大学生物冶金教育部重点实验室,长沙410083

出　　处：《Transactions of Nonferrous Metals Society of China》2011年第6期1383-1387,共5页中国有色金属学报（英文版）

基　　金：Project(50774102)supported by the National Natural Science Foundation of China;Project(2004CB619204)supported by the National Basic Research Program of China

摘　　要：To screen the high efficient mixed culture and understand the bioleaching behaviors of mixed culture for low-grade copper sulfide ore bioleaching,ten mixed cultures were collected and screened from different acid mine drainages obtained from sulfide mines of China.The leaching rate was set as criterion to screen the mixed culture and the metagenomic approach.Community genome array（CGA） was used for analyzing the mixed culture microbial community shift during the bioleaching process.The results indicate that the mixed culture obtained from Yinshan（YS） lead-zinc mine in Dexing of Jiangxi province in China reaches the maximum copper extraction（68.89%） during the one bioleaching period of 24 d.CGA results show that YS culture contains nine kinds of bacteria which are belong to six divisions,and the microbial community structure is changing during the bioleaching process.This provides a good way to accelerate the bioleaching process and reveals the microbial community shift during the bioleaching process.为了筛选可高效浸出低品位硫化矿的混合菌株,从10个典型的硫化矿矿区的酸性矿坑水中分离富集到混合菌株。以浸出率作为筛选的主要标准,筛选后的高效菌株,利用群落基因组芯片分析混合菌群的组成和过程变化。浸矿持续进行24d后,采集自江西德兴银山铅锌矿的混合菌浸出率最高,为68.89%。群落基因组芯片结果表明银山菌群包含9种菌,可被分为6类,在浸出过程中群落始终在变化。该研究为加速浸出和了解浸出过程中群落演替提供了一个较好的方法。

关 键 词：BIOLEACHING high efficient mixed culture community genome array（CGA） microbial community shift 

分 类 号：TD925.5[矿业工程—选矿]