替代对照品法测定淫羊藿中淫羊藿苷的含量  

Determination of Icariin Contents in Epimedium brevicornum Maxim.with Substitute Reference Substance

作　　者：李莉[1] 郝小燕[1] 刘昌福[1] 肖海涛[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《贵阳医学院学报》2011年第3期246-250,共5页Journal of Guiyang Medical College

基　　金：贵州省科学技术基金项目(合同号:20082153);贵阳市科技局"产学研"技术项目(合同号:2008-24-2)

摘　　要：目的:建立HPLC替代对照品法测定淫羊藿中淫羊藿苷的含量。方法:选取芦丁作为淫羊藿中淫羊藿苷的替代对照品,在不同的条件下测定替代对照品相对对照品的校正因子,利用校正因子和替代对照品芦丁进行淫羊藿中淫羊藿苷的含量测定。结果:淫羊藿苷和芦丁进样量分别在0.13～3.20μg(r=0.999 6)和0.34～8.50μg(r=0.999 7)内与峰面积呈良好的线性关系(n=6),测得校正因子f=2.586 1,用替代对照品测定法测得回收率为96.01%(n=9),RSD=1.42%。结论:在高效液相色谱仪上用替代对照品测定淫羊藿中淫羊藿苷的含量是可行的、实用的。Objective：To establish a high performance liquid chromatography （HPLC） substitute reference method for determination of icariin in Epimedium brevicornum Maxim.Methods：Rutin was chosen as substitute reference substance.Correction factor was obtained by measuring icariin and rutin contents in Epimedium brevicornum,reference substance,and substitute reference substance respectively under different conditions.Icariin contents of Epimedium brevicornum was determined with substitute reference substance and the correction factor.Results：The calibration curve for icariin and rutin were linear in the range of 0.13～3.20 μg（r=0.999 6） and 0.34～8.50 μg（r=0.999 7） respectively.The corection factor（f） was 2.586 1.The average recovery was 96.01%（n=9）,and RSD was 1.42%.Conlusions：This method is feasible and pragmatic.

关 键 词：替代对照品 校正因子 芦丁 淫羊藿 

分 类 号：R284.1[医药卫生—中药学]