流感病毒H1N1感染后宿主细胞MTH1基因表达的研究  

Study on the expression of MTH1 gene in MDCK cells infected by influenza A virus

作　　者：马红梅[1] 郑君德[1] 肖洪广[1] 刘彦明[2] 蒋月婷[1] 李伟强[3] 罗嘉莉[3] 曹顺旺[3] 

机构地区：[1]广州医学院第一附属医院检验科,广东广州510120 [2]粤北人民医院检验科,广东韶关512026 [3]广州医学院检验系,广东广州510182

出　　处：《热带医学杂志》2011年第6期617-619,共3页Journal of Tropical Medicine

基　　金：广东省自然科学基金博士科研启动项目(9451018201003654);广州医学院博士启动基金(2008C17);粤教科[2007]26号第八轮广东省高等学校重点扶持学科资助项目

摘　　要：目的观察H1N1型流感病毒诱导的MDCK细胞抗氧化相关修复基因MTH1表达量的变化。方法 MDCK细胞培养后,H1N1型流感病毒感染0、1、3、6、12、24、48h,在各时间点经RT-PCR反应检测细胞内MTH1基因的相对表达量。结果甲型H1N1流感病毒感染后0、6、12h,MTH1基因的表达量与正常对照组差异无统计学意义;感染后1、3hMTH1基因的表达量显著高于正常对照组;感染后24、48h表达量显著低于正常对照组。结论 H1N1流感病毒感染细胞的MTH1基因表达的增加,可能增加细胞对DNA氧化损伤的修复能力,在流感发生和防御机制中起到作用。Objective To observe the expression changes of antioxidant-retated repair genes MTH1 in the H1N1 influenza virus infected MDCK cell. Methods MDCK cells were cultured and infected with H1N1. The relative expression of MTH1 gene of the host cells were detected by the RT-PCR after being infected for 0,1,3,6,12,24 and 48 hours. Results There were no significant differences of the MTH1 gene expression between the experimental groups and the control groups for the 0, 6 and 12 hours,but was significantly higher than the control group after infected for 1 h,and 3 h,and expression was significantly lower than the control group after infected for 24 h, and 48 h. Conclusion The expression of MTH1 gene H 1N1 was increased in influenza virus infected cells.Increased expression of MTH1 gene may increase the cells ability to repair DNA oxidative damage. This may play a role in defense of influenza.

关 键 词：甲型H1N1流感病毒 MTH1基因 RT-PCR 基因表达 

分 类 号：R511.7[医药卫生—内科学]