GM6001对兔LASEK术后角膜上皮下雾状混浊形成的影响  

Effects of GM6001 on haze formation after excimer laser epithelial keratomileusis in rabbit

作　　者：陈晓玲[1] 高晓唯 胡裕坤 郭云林 李晓虹 李文静 

机构地区：[1]石河子大学医学院,832002 [2]解放军第四七四医院全军眼科中心,乌鲁木齐830013

出　　处：《中华实验眼科杂志》2011年第7期631-634,共4页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景近年来基质金属蛋白酶（MMPs）在准分子激光术后创伤修复过程中的作用越来越受到学者们的关注。目的探讨MMPs抑制剂GM6001对兔准分子激光角膜上皮下磨镶术（LASEK）术后角膜上皮下雾状混浊（haze）形成的影响。方法27只新西兰白兔双眼行一10．00D激光切削的LASEK，手术后动物被随机分为GM6001组、氟米龙组和阴性对照组，术后分别用150Ixmol／L的GM6001滴眼液、质量分数0．1％氟米龙和氧氟沙星滴眼液点眼。每天在裂隙灯下对兔LASEK术后角膜上皮下haze进行观察和分级。分别于术后2、4、8周各组随机选取6只眼行角膜共焦显微镜检查，制备角膜组织切片行组织病理学检查，在透射电子显微镜下行角膜组织的超微结构检查。结果LASEK术后2周和4周，阴性对照组haze分级较高的眼数多于GM6001组和氟米龙组，差异均有统计学意义（P〈0．05），GM6001组和氟米龙组haze分级水平均明显低于阴性对照组（P〈0．01），但GM6001组和氟米龙组相比，差异无统计学意义（P〉0．05）；术后8周组3个组间haze分级的总体比较差异无统计学意义（P〉0．05）。术后2、4、8周GM6001组术区前部基质内角膜成纤维细胞数均较对照组和氟米龙组减少，差异均有统计学意义（P〈0．05），各时间点对照组和氟米龙组差异无统计学意义（P〉0．05）。LASEK术后GM6001组和氟米龙组兔角膜上皮细胞的形态改变、胶原纤维排列的紊乱情况均轻于阴性对照组。结论GM6001通过抑制LASEK术后MMPs对角膜基质的降解，从而抑制角膜基质成纤维细胞的增生及胶原纤维的合成，减少haze的形成，其疗效与氟米龙相似。Background More and more attentions are paid to the effect of matrix metalloproteinases （MMPs） in the process of wound healing after excimer laser. Objective The present study was to investigate the effects of GM6001, a matrix metalloproteinases inhibitor, on haze formation after excimer laser epithelial keratomileusis （LASIK）. Methods LASEK of -10. 00 diopter was bilaterally performed on 27 New Zealand white rabbits and then divided randomly into the GM6001 group,Fluorometholone （FML） group and normal control group. Corneal haze was graded under dim light at 2 weeks ,4 weeks and 8 weeks after operation, and the number of eyes in different grades of haze were compared among the three groups. Six corneal samples were harvested in each group for eonfocal microscope examination at 2,4,8 weeks. The corneal histopathological examination was carried out with the optical microscope,and the ultrastructure of the cornea was examined under the transmission electron microscope. Results The numbers of eyes of different corneal grades of haze at 2,4 or 8 weeks after surgery were significantly different among the three groups （P〈0. 05）. Compared with the normal control group,the corneal haze grades in the GM6001 group and FML group were apparently lower （P〈0. 01 ） , but there was no significant difference in the numbers of eyes of different corneal grades of haze between the GM6001 group and FML group （P〉0.01）. The numbers of keratocytes in the GM6001 group were apparently lower than those in the normal control group and FML group in 2,4 or 8 weeks after operation （ P〈0. 05 ）. No significant difference in the keratocytes was seen between the normal control group and FML group at various time points （P〉0.05）. Corneal epithelial cell morphology, disordered arrangement of collagen fibers in GM6001 group and in FML group were less pronounced than in the negative control group. Conclusion GM6001 has an inhibitory effect on the formation of corneal haze after LASEK which suppresses prolifera

关 键 词：角膜 基质金属蛋白酶 基质金属蛋白酶抑制剂 准分子激光角膜上皮下磨镶术 角膜上皮下混浊 

分 类 号：R779.63[医药卫生—眼科]