甘草甜素减轻博莱霉素致大鼠肺纤维化  

Glycyrrhizin relieves pulmonary fibrosis by Bleomycin in rats

作　　者：殷宗宝[1] 李英[1] 邓超[1] 曹玉芳[1] 

机构地区：[1]海口市人民医院重症医学科,海南海口570208

出　　处：《基础医学与临床》2011年第8期905-908,共4页Basic and Clinical Medicine

摘　　要：目的探讨甘草甜素减轻博莱霉素致大鼠肺纤维化的机制。方法将大鼠随机分成对照组、博莱霉素致大鼠肺纤维化模型组、地塞米松(DXM)、甘草甜素低、中及高剂量治疗组。注博莱霉素后第28天,连续14 d同一时间段腹腔注射。第15天取左肺下叶供免疫组化检测TGF-β1、IFN-γ表达;右肺下叶供病理组织观察。用ELISA双抗体夹心法测定各组大鼠血清IL-4、IFN-γ含量。结果 (1)模型组大鼠肺组织成纤维细胞反应增强,TGF-β1染色及定量表达增强;而甘草甜素各组、DXM组减轻明显(P<0.01);(2)模型组大鼠血清IL-4含量较高,IFN-γ含量较低;甘草甜素各组及DXM组则相反(P<0.05)。(3)甘草甜素各组、DXM组肺组织IFN-γ染色及定量表达增强,甘草甜素低剂量组较其他组增强(P<0.01)。结论甘草甜素通过降低肺组织TGF-β1表达和血清IL-4含量,上调IFN-γ表达,提高血清IFN-γ含量,从而减轻肺纤维化程度。Objective To investigate effects of glycyrrhizin in rat model of pulmonary fibrosis induced by bleomycin.Methods Sprague Dawley rats were randomly divided into pulmonary fibrosis group,NS group,DXM,glycyrrhizin low dose,meta-dose,high dose treatment group.All pulmonary fibrosis rats in the treatment group were injected traperitoneally after 28 days and lasted for 14 days.At the 15th days,the left lung was measured by immunity class TGF-β1,IFN-γ;the right lung tissue was sliced and cell apoptosis was detected.The blood-serum was measured by ELISA IFN-γ and IL-4.Results（1）The pulmonary alveolitis and fibrosis of BLM group were severe.TGF-β1 protein expression in bronehiolar epitheliums of BLM group,was higher and more significantly deviated（P0.01）.（2）IL-4 level of BLM group was higher than all the treatment groups,but the IFN-γ level was an exception（P0.05）.（3）Strong IFN-γ protein expression was detected in glycyrrhizin treatment groups.IFN-γ protein expression in glycyrrhizin low dose group was more significant deviation than in the other group（P0.01）.Conclusions The glycyrrhizin decreased TGF-β1 protein expression and IL-4 level of pulmonary fibrosis rats,but IFN-γ was an exception.Glycyrrhizin has strong ability to treat antifibrosis.

关 键 词：肺纤维化 干扰素Γ 甘草甜素 转化生长因子Β1 白细胞介素4 

分 类 号：R285.5[医药卫生—中药学]