瑞芬太尼诱导大鼠脊髓背角痛觉过敏及利多卡因的抑制作用  

Remifentanil-induced hyperalgesia in spinal dorsal horn and the inhibition of lidocaine in rats

作　　者：崔伟华[1] 谭红[2] 韩如泉[1] 李树人[2] 李俊发[3] 

机构地区：[1]首都医科大学附属北京天坛医院麻醉科,北京100050 [2]首都医科大学附属北京友谊医院麻醉科,北京100050 [3]首都医科大学神经生物学系,北京100069

出　　处：《基础医学与临床》2011年第8期914-918,共5页Basic and Clinical Medicine

摘　　要：目的探讨大鼠脊髓背角细胞蛋白激酶Cγ(PKCγ)膜转位/激活在瑞芬太尼诱导痛觉过敏中的作用及利多卡因的抑制作用。方法将大鼠随机分为4组:(1)丙泊酚组(P组),(2)瑞芬太尼组(R组),(3)瑞芬太尼-利多卡因组(RL组)及(4)利多卡因组(L组)。比较4组麻醉后累积疼痛评分和机械性刺激缩足阈值。用免疫印迹(每组n=8)和免疫荧光法测量脊髓背角PKCγ膜转位/激活。结果 (1)累积疼痛评分P、RL和L组相似;R组18(15-20.75)高于P组11.5(8-13.5)、RL组11(9-14)和L组8.5(11-16.25)(P<0.05)。(2)手术侧机械性刺激缩足阈值R组39.2(39.2-68.6)mN低于P组117.6(58.8-137.2)mN、RL组588(588-588)mN和L组588(588-588)mN组(P<0.05)。(3)蛋白免疫印迹显示脊髓背角PKCγ膜转位/激活R组(假手术组153%±35%,手术对侧为160%±41%,手术侧为157%±36%)高于其他3组,R组PKCγ免疫阳性显色在细胞边缘增强。术后短时间内,未发现组织损伤对脊髓背角PKCγ膜转位/激活的影响。结论脊髓背角PKCγ膜转位/激活参与了瑞芬太尼诱导的痛觉过敏,后者可被利多卡因抑制。Objective To determine the involvement of conventional protein kinase Cgamma（cPKCγ） in the inhibitory action of lidocaine on remifentanil-induced hyperalgesia of rats after propofol-remifentanil-based anesthesia.Methods Male Sprague-Dawley rats were allocated into the following groups randomly： propofol only（group P）,propofol＋remifentanil（group R）,propofol＋remifentanil＋lidocaine（group RL）,and propofol＋lidocaine（group L）.Cumulative pain score and withdrawal response to mechanical stimulation,immunoblotting,and immunofluorescence were applied to observe remifentanil-induced hyperalgesia and cPKCγ membrane translocation.Results（1）Cumulative pain score in group R was higher than that in other groups on postanesthesia 120 min（P0.05）.While it were similar among group P,RL and L.（2）Withdrawal threshold on the ipsilateral side to incised plantar in group R were lower than the other groups（P0.05） on postanesthesia 120 min.（3）Both immunoblotting and immunofluorescence demonstrated that the membrane translocation of PKCγ in dorsal horn neurons increased in propofol-remifentanil anesthetized animals and systemic lidocaine inhibited it.Surgery procedure won＇t affect the membrane translocation of PKCγ in dorsal horn neurons shortly after anesthesia.Conclusions Increased PKCγ membrane translocation in spinal dorsal horn involves in remifentanil-induced hyperalgesia,which was inhibited by systemic lidocaine and may contributes to reduction of postoperative pain in rats after remifentanil-based anesthesia.

关 键 词：瑞芬太尼 利多卡因 痛觉过敏 蛋白激酶CΓ 脊髓背角 

分 类 号：R965[医药卫生—药理学]