CD74基因对重症肌无力患者胸腺上皮细胞IFN-γR基因表达的影响  

Effects of CD74 gene on IFNγR gene expression in MG TEC

作　　者：李倩如[1] 臧文巧[1] 高峰[2] 杜英[1] 张清勇[3] 

机构地区：[1]郑州大学基础医学院微生物学与免疫学教研室,河南郑州450001 [2]河南省医学科学研究所,河南郑州450052 [3]郑州大学第二附属医院胸外科,河南郑州450000

出　　处：《细胞与分子免疫学杂志》2011年第7期730-732,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30570625);河南省自然科学基金资助项目(511042700)

摘　　要：目的:探讨CD74基因对重症肌无力患者胸腺上皮细胞(TEC)IFN-γR基因表达的影响。方法:原代培养重症肌无力患者胸腺上皮细胞,脂质体法转染pCMV-CD74,荧光定量RT-PCR法检测转染结果及TEC中IFN-γR等与自身免疫病相关基因mRNA水平的表达。结果:原代培养并传代的胸腺上皮细胞转染pCMV-CD74质粒后,CD74基因的表达显著提高。TEC高表达CD74基因,可引起HLA-A、ECFG1、IL-4R、IL-32和IFN-γR基因表达显著增加。结论:CD74基因对TEC表达IFNγR基因有明显的促进作用。AIM：To study the effects of CD74 gene on IFN-γR expression in myasthenia gravis thymic epithelial cell（TEC）.METHODS：PCMV-CD74 transiently transfect to primary cultured TEC mediated with lipofectamine,the result of transfection and some autoimmune related genes such as IFN-γR were detected by real-time RT-PCR.RESULTS：Real-time quantitative RT-PCR results show that the mRNA expression level of IL-4R,IL-32,IFN-γR,ECGF1,HLA-A,HLA-DR increased significantly,with the high express ion of CD74 gene in thymic epithelial cells.CONCLUSION：CD74 gene over-expression in TEC can increase IFN-γR mRNA expression.

关 键 词：CD74 胸腺上皮细胞 IFN-γR 自身免疫相关基因 

分 类 号：R392.11[医药卫生—免疫学]