丙型肝炎病毒NS_3蛋白C末端细胞毒T细胞表位的动力学分析  

Long-term evolution of C-terminal of HCy NS_3 protein containing the predicted CTL epitopes in two HCV patients

作　　者：汪涛[1] 郭华章[1] 王国力[2] 齐连权[3] 徐俊杰[3] 王海涛[3] 金伯泉[1] 

机构地区：[1]第四军医大学基础部,西安710032 [2]军事医学科学院生物工程研究所,北京710032 [3]军事医学科学院微生物流行病研究所,710032

出　　处：《中华肝脏病杂志》1999年第4期207-210,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金!39630020

摘　　要：目的研究丙型肝炎病毒（ＨＣＶ）ＮＳ３蛋白Ｃ末端细胞毒Ｔ细胞（ｃｙｔｏｔｏｘｉｃＴｌｙｍｐｈｏｃｙｔｅ，ＣＴＬ）表位的长期演变规律。方法血清学法检测了２例慢性ＨＣＶ感染者的ＨＬＡ分型，并以ＨＬＡ结合基序为依据，预测了ＨＬＡ限制的ＣＴＬ表位。血清样本中的ＨＣＶ基因片段用ＰＣＲ扩增，Ｓａｎｇｒｒ法测定核苷酸序列，并翻译成蛋白质。用计算机软件分析了ＣＴＬ表位的改变。结果５年和３年内预测的ＨＬＡ—Ａ２和ＨＬＡ—Ａ１１限制的ＣＴＬ表位在２例携带者中都未发生改变。结论ＨＣＶＮＳ３蛋白Ｃ末端的ＣＴＬ表位可能与ＨＣＶ慢性化无关。Objective To investigate the Long-term evolution of NS3 C-terminal protein containingthe predicted CTL epitopes. Methods The HLA typing was determined serologically in two chronic HCVpatients. Batal on this, the HLA restricted CTL epitopes were predicted by HLA binding motif. SerasamPles were amplified by PCR, HCV was sequenced and translated into proteins. The chang6s of predicted CTL opitopes were analysed. Results No obvious chance was oberserved in predicted CTL epitopesin two Chronic HCV patients during 5 and 3 years. ConcluSion it demonstrated that the C-terminal ofHCV NS3 protein containing the predicted CTL epitopes may have no relationship with HCV persistence.

关 键 词：丙型肝炎病毒 抗原决定簇 T淋巴细胞 细胞毒性 

分 类 号：R373.21[医药卫生—病原生物学]