老年鼠六种组织细胞的细胞核与染色质DNaseⅠ的消化敏感性研究  

DNaseⅠSensitivity of Nucleus and Chromatin in Six Different Tissues of Old Rates

作　　者：胡火珍[1] 邢一玲[1] 李小玉[1] 应炎斌[1] 肖时超 郑雪莲[1] 

机构地区：[1]华西医科大学基础医学院医学生物学与细胞生物学教研室,成都610041

出　　处：《华西医科大学学报》1999年第4期405-407,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金!(批准号39470763)

摘　　要：为探讨衰老的机理，作者研究了老年鼠和断奶乳鼠的心、肝、脾、肺、肾、脑等６ 种组织细胞的细胞核和染色质对ＤＮＡ 酶Ⅰ（ＤＮａｓｅⅠ）的消化敏感性。结果显示：①老年鼠的心、肝、脾、肺、肾、脑等６种组织细胞的细胞核和染色质对ＤＮａｓｅⅠ的消化敏感性均较断奶乳鼠相应的组织低；②老年鼠的心、肝、脾、肺、肾、脑等６ 种组织的消化敏感性各不相同，脑组织的消化敏感性最低（Ｐ＜ ０．０１）；③断奶乳鼠脑组织的消化敏感性也较其它５ 种组织低，但乳鼠中其它５ 种组织细胞核及染色质的消化敏感性差异不明显。作者认为，老年鼠各组织细胞核和染色质转录活性降低，可能与活性基因减少有关。In order to investigate the mechnism of aging, we studied the DNase Ⅰ sensitivity of nucleus and chromatin in six different tissuse (heart, liver, spleen, lung, kidney and brain of old and weanling wistar rats. The results showed that the DNase Ⅰ digestive sensitivity levels of nucleus and chromatin in the tissuse of the old rats were lower than those of the weanling rats repectively that in the old rats, the DNase Ⅰ digestive sensitively in the tissuse varied, the lowest sensitivity was in brain ( P <0.01); and that in the weanling rats, lowest DNase Ⅰ digestive sensitvity of nucleus and chromatin was also in the brain, but the difference was not significant.

关 键 词：细胞核 染色质 DNA酶Ⅰ 消化敏感性 衰老 

分 类 号：R339.38[医药卫生—人体生理学] Q419[医药卫生—基础医学]