精氨酸加压素对脓毒症大鼠的肝肾保护作用  

The Protection Effect of Arginine Vasopressin on the Function of Organ in the Septic Rat

作　　者：杨磊[1] 李晓云[2] 柴琛[1] 乔玮[1] 鲁彦[2] 曹农[1] 

机构地区：[1]兰州大学第一医院普外一科,730000 [2]中国人民解放军第一医院检验科,兰州730030

出　　处：《医学研究杂志》2011年第7期30-32,共3页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30700266);陕西省卫生厅资助项目(06E05);兰州大学中央高校基本科研业务费专项资金(lzujbky-2010-146)

摘　　要：目的探讨精氨酸加压素(AVP)对毒症大鼠的肝、肾的保护作用。方法雄性SD大鼠通过盲肠结扎穿刺术(CLP)制作脓毒症大鼠模型,以假手术组(n=4)为对照,部分CLP大鼠(n=18)术后4h、8h、12h测肝、肾功能生化指标,部分大鼠CLP术后8h通过侧脑室注射AVP的方式中枢补充AVP(n=6)或人工脑脊液(aCSF,n=4),另一部分大鼠CLP术后6h通过静脉持续泵入AVP(n=6)或生理盐水(n=4)2h,分别在给药后1h测肝、肾功能生化指标变化;结果CLP术后4 h后大鼠血浆ALT、AST、ALP、BUN及Cr开始升高,CLP术后8h有统计学意义,后ALT、AST、ALP、BUN及Cr逐渐升高,CLP术后6h静脉持续泵入AVP[2ng/(kg.h)]2h与静脉泵入生理盐水2h组相比大鼠血浆ALT、AST、ALP、BUN及Cr明显降低;但CLP术后8 h侧脑室给予AVP后大鼠血浆ALT、AST、ALP、BUN及Cr与对照组比较明显升高。结论脓毒症大鼠肝肾功能受损可能与血浆AVP水平有关,早期静脉给予AVP可改善肝肾功能,具有器官保护作用。Objective To explore the protecting effects of arginine vasopressin （AVP） on organic function in septic rat. Methods Sepsis rat model was induced in 18 rats by cecal ligation and punctureinduced （CLP）. Levels of ALT, AST, ALP, BUN and Cr were detected in 4 sham rats and in CLP rats after 4 - 12h. In another 20 CLP rats, AVP was supplemented by continuous intravenous infusion or intracerebroventricular injection before these parameters were detected. Results Sepsis produced a gradual increase in ALT, AST, ALP, BUN and Cr from 4h after CLP. Compared with sham group, continuous infusion of AVP at a rate of 2ng/（ kg · h） for 6 hours produced a profound decrease in ALT, AST, ALP, BUN and Cr. However, injection of AVP （50ng） into lateral ventricles（LV） for 8 hours after CLP caused an increase in ALT,AST,ALP,BUN and Cr. Conclusion It shows that the function of liver and renal has relation to plasma vasopressin levels in the rats with endotoxemia. Continuous infusion of AVP at the initial phase of septic shock can protect some organs function.

关 键 词：精氨酸加压素 脓毒症 肝脏 肾脏 

分 类 号：R364.6[医药卫生—病理学]