慢性乙型肝炎患者、乙型肝炎肝硬化患者外周血CD4^+CD25^+调节性T细胞的水平及意义  

The Level of CD4^+CD25^+ Regulatory T Cells in Peripheral Blood in Patients with Chronic Hepatitis B and HBV-related Liver Cirrhosis

作　　者：张磊[1] 谷甸娜[1] 陈永平[1] 郑明华[1] 

机构地区：[1]温州医学院附属第一医院感染内科,325000

出　　处：《医学研究杂志》2011年第7期60-62,共3页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y207464);浙江省科技厅新苗人才计划项目(2008R40G2090022)

摘　　要：目的测定慢性乙型肝炎患者、乙型肝炎肝硬化患者外周血CD4+CD25+调节性T细胞(Treg)的水平并探讨其意义。方法流式细胞术检测12例慢性乙型病毒性肝炎患者,15例乙型病毒性肝炎后肝硬化患者和6例健康对照者外周血中CD4+CD25+Treg的频率。结果 12例慢性乙型病毒性肝炎患者外周血中CD4+CD25+Treg占CD4+淋巴细胞的比例为(12.21±1.46)%,15例慢性乙型病毒性肝炎后肝硬化患者外周血中CD4+CD25+Treg占CD4+淋巴细胞的比例为(11.36±1.06)%,与6例健康对照组(9.86±1.00)%相比差异有显著性(P<0.05),慢性乙型病毒性肝炎与慢性乙型病毒性肝炎后肝硬化组间比较差异无显著性(P>0.05)。结论 Treg与CHB患者持续HBV感染密切相关,而是否参与乙肝后肝硬化的发病机制有待进一步研究。Objective To analyze the level of CD4 ＋ CD25 ＋ regulatory T cells（Treg） in peripheral blood of chronic HBV and HBV - related liver cirrhosis patients and study its clinical significance. Methods 12 CHB patients, 15 HBV - related liver cirrhosis patients and 6 healthy controls were enrolled in the study. The flow eytometry was used to detect the frequency of CD4 ＋ CD25 ＋ Tregs in the peripheral blood. Results The frequency of CD4 ＋ CD25 ＋ Tregs in the peripheral blood in the CHB and cirrhosis were （ 12.21±1. 46） % and （ 11.36± 1.06 ） % respectively,which were both higher than those （9.86 ± 1.00） % of the controls （ P 〈 0.05 ）. There was no significant difference hetwwen the CHB and cirrhosis（P 〉0.05）. Conclusion These findings suggest that CD4 ＋ CD25＋ Treg plays an role in the persistent infection of HBV ,but is not sure in the mechanism of HBV -related liver cirrhosis.

关 键 词：CD4+CD25+调节性T细胞慢性 乙型病毒性肝炎 肝硬化 

分 类 号：R392.4[医药卫生—免疫学]