p47phox向胞膜移位调节高糖导致的内皮细胞内活性氧升高  

Reactive Oxygen Species is Regulated by P47phox in Human Umblical Vein Endothelical Cells Cultured with High Concentration of Glucose

作　　者：王蕾[1] 吴和平[1] 舒筱灿[1] 舒旭[1] 伍世挥[1] 罗海[1] 

机构地区：[1]湖南怀化医学高等专科学校基础医学部,418000

出　　处：《医学研究杂志》2011年第7期65-67,共3页Journal of Medical Research

基　　金：湖南省教育厅科研基金资助项目(07C057)

摘　　要：目的探讨在高浓度葡萄糖刺激下NADPH氧化酶亚单位p47phox调节内皮细胞内活性氧升高的机制。方法Ⅱ型胶原酶法分离人脐静脉内皮细胞,流式细胞仪检测细胞内活性氧的生成量,western blotting和免疫荧光分别检测细胞内p47phox蛋白的表达和其细胞内的定位。结果高浓度葡萄糖刺激HUVECs内活性氧的升高;NADPH氧化酶抑制剂DPI及apo-cynin抑制高糖导致的活性氧升高;高糖刺激状态下p47phox蛋白表达量并不增加,它由胞质向胞膜移位,DPI和apocynin抑制p47phox移位。结论 p47phox通过向胞膜移位调节高浓度葡萄糖导致的HUVECs内NADPH氧化酶源性的活性氧升高。Objective To investigate how reactive oxygen species （ROS） generation was regulated by p47phox, subunit of NADPH oxidase （NOX） , in human umbilical vein endothelial cells （HUVECs） cultured with high concentration of glucose. Methods Human umbilical vein endothelial cells was isolated by collagenase I] ~ Flow cytometry was used to detect intracellular ROS production. Western blotting and immunofluorescence were used to investigate the expression and location of p47phox protein in HUVECs, respectively. Results High concentration of glucose induced ROS generation in HUVECs. DPI and apocynin attenuated ROS production, p47phox was not upregulated but translocated from cytoplasm to cell membrane in the state of high glucose, p47phox translocation was suppressed by DPI and apocynin. Conclusion High glucose can induce ROS generation by means of translocation of p47phox from cytoplasm to cell membrane in HUVECs.

关 键 词：p47phox 高糖 活性氧 内皮细胞 

分 类 号：R285.5[医药卫生—中药学]