猪PEG1基因多态性及其遗传印记  

Studies on SNP and genomic imprinting of the PEG1 gene in swine

作　　者：李莉[1] 贾同[1] 吴同山 张守全[1] 

机构地区：[1]华南农业大学动物科学学院,广东省农业动物基因组学与分子育种重点实验室,广州510642 [2]东莞市畜牧科学研究所,东莞523086

出　　处：《遗传》2011年第7期738-742,共5页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划(973计划)项目(编号:2010CB945001;2011CB944202)资助

摘　　要：PEG1基因影响动物胚胎生长及母性行为,多数动物PEG1为父方表达的遗传印记特征,但出生后猪的PEG1印记表达尚不清楚。因此,文章选取长白、大白和蓝塘3个品种共166头纯种猪,在猪的PEG1基因外显子12区域内寻找SNP,采用PCR-SSCP方法对其多态性进行检测和基因频率分析;取带有PEG1基因该位点SNP为杂合的仔猪3头,对其胃、胸腺、胰、脾、肺、肌肉、肝、舌、肾、脑、膀胱、心脏等组织器官和胎盘的mRNA产物分别进行RT-PCR-SSCP分析,结果表明:PEG1基因外显子12存在一个由G突变为A的单核苷酸多态性位点;PEG1的外显子12在3头仔猪的主要组织器官仅表达父亲来源的等位基因,表明猪的PEG1基因呈母方印记、父方表达的遗传特征。PEG1 affects animal embryo growth and maternal behavior.The paternal allele-specific expression of PEG1 was reported in most animal species,but the expressive pattern of PEG1 was not clear in piglets born.In order to investi-gate the imprinting pattern of PEG1 in pig,166 samples were used to SNP of PEG1 from Landrace,Yorkshire,and Lantang breeds by PCR-SSCP.Single nucleotide polymorphism（SNP） of PEG1 in exon 12 was identfied.The genotype frequency and the expressive pattern were anslyzed in swine by RT-PCR-RFLP/SSCP.One SNP（a G→A transition） was identified in exon 12 of PEG1.Maternal expression of PEG1 exon 12 was observed in all major organs（stomach,thymus,pancreas,spleen,lung,muscle,liver,tongue,kidney,brain,bladder,and heart） and placenta of three heterozygous pigs.PEG1 was paternally expressed and maternally imprinted in swine.

关 键 词：猪 PEG1 单核苷酸多态性(sNP) 遗传印记 

分 类 号：Q78[生物学—分子生物学] S828[农业科学—畜牧学]