马铃薯晚疫病菌全基因组分泌蛋白的初步分析  

Genome-wide analysis of the secreted proteins of phytophthora infestans

作　　者：周晓罡[1,2] 侯思名[3] 陈铎文[1,2,4] 陶南[1,2] 丁玉梅[1,2] 孙茂林[1,2] 张绍松[1,2] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,昆明650223 [2]云南省农业生物技术重点实验室,昆明650223 [3]昆明学院生命科学与技术系,昆明650031 [4]云南大学生命科学学院,昆明650091

出　　处：《遗传》2011年第7期785-793,共9页Hereditas（Beijing)

基　　金：云南省十一五科技攻关项目(编号:2006NG08);国家自然科学基金项目(编号:31060021);云南省自然科学基金项目(编号:2006C0062M;2008ZC100M)资助

摘　　要：利用马铃薯晚疫病菌全基因组测序结果,结合计算机技术和生物信息学的方法,对马铃薯晚疫病菌的蛋白进行分析,为明确该病原菌与寄主互作的分子机制奠定基础。文章应用信号肽预测软件SignalP v3.0和PSORT,跨膜螺旋结构预测软件TMHMM-2.0和THUMBUP,GPI锚定位点预测软件big-PI Predictor,亚细胞器中蛋白定位分布预测软件TargetP v1.01,对已经公布的马铃薯晚疫病菌全基因组22 658个蛋白质氨基酸序列进行分析。结果发现,晚疫病菌全基因组编码蛋白中有671个为潜在的分泌型蛋白,占编码蛋白总数的3.0%。其中有45个分泌蛋白有功能方面的描述,其功能涉及细胞代谢、信号转导等方面;此外,还有一些与激发子类似的分泌蛋白,它们可能与晚疫病菌的毒性有关。Based on the Phytophthora infestans genome sequence,we used bioinformatics and computer-based prediction algorithms to predict the secreted proteins of P.infestans in detail,which would help us to elucidate the molecular mecha-nism underlying the interaction between the host plants and the P.infestans.In this study,the signal peptide prediction algo-rithms SignalP v3.0 and PSORT,transmembrane helix prediction algorithms TMHMM-2.0 and THUMBUP,GPI-anchoring site prediction algorithm big-PI Predictor,and subcellular protein location distribution algorithm TargetP v1.01 were used to analyze the 22658 protein sequences of P.infestans published.Our results suggested that there might be 671 secreted pro-teins,accounting for 3.0% of the total proteins.Among them,the functions of the 45 secreted proteins had been described previously.Their functions involved cellular metabolism and signal transduction etc.In addition,some of the secreted pro-teins were functionally similar to elicitin,which were likely to be associated with the virulence of P.infestans.

关 键 词：晚疫病菌 信号肽 分泌蛋白 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]