扇贝养殖海区中小型浮游生物携带AVNV的荧光定量检测  

Identification by FQ-PCR of AVNV carried by mesoplankton and microplankton at two ocean sites for culturing Chlamys farreri

作　　者：蔡玉勇[1,2] 任伟成[1] 曲朋[1,2] 贾志磊[1,2] 王崇明[1] 李赟[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]中国海洋大学,山东青岛266003

出　　处：《中国水产科学》2011年第4期735-741,共7页Journal of Fishery Sciences of China

基　　金：国家863计划项目(2006AA100307);现代农业产业技术体系建设专项资金资助(nycytx-47)

摘　　要：为了解中小型浮游生物与扇贝急性病毒性坏死症（AVND）的流行传播关系,于2009年对青岛流清河与荣成桑沟湾2个不同扇贝养殖海区的浮游生物进行了采样调查,利用荧光定量PCR（Fluorescent quantitative PCR,FQ-PCR）技术对所采集样品携带急性病毒性坏死病毒（AVNV）的情况进行了定量检测。结果表明,2个扇贝养殖海区的浮游生物样品均检测到AVNV,而且不同粒径的浮游生物携带AVNV载量有差异（P〈0.05）：小于200目粒径的浮游生物携带AVNV载量最大,达到9.45×106 copy/mg（DNA）;60~200目粒径的浮游生物次之,大于60目粒径的浮游生物携带量最少,仅为5.81×104 copy/mg（DNA）。在上述2个扇贝养殖海区,均在8月份检测到浮游生物携带AVNV。该结果与栉孔扇贝夏季大规模死亡在时间上是吻合的。结论认为,中小型浮游生物可以携带AVNV,并有可能在扇贝急性病毒性坏死症的流行和传播中起到传播媒介的作用。This paper describes the relationship between transmission routes of AVNV and mesoplankton and mi-croplankton in the ocean for culturing scallops.Plankton samples were collected in Liuqinghe Qingdao and Sang-gou Bay,Rongcheng,from April to November 2009.In August,the AVNV copy number carried by plankton was identified by FQ-PCR.Results showed that plankton samples from both ocean areas carried the virus,and that the virus numbers differed with different particle sizes.Plankton samples with the same particle sizes at the Liuqinghe site carried more AVNV copy numbers than those at the Sanggou Bay site at a given time.Plankton particle sizes smaller than 200 mesh carried the greatest amount of viruses（viral DNA number reached 9.45×106 copy/mg DNA）,followed by plankton particle sizes between 60？200 mesh size.Plankton particle sizes larger than 60 mesh carried the least amount viruses.Finally,results suggest that plankton can carry AVNV,and that they may be an important host of AVNV.

关 键 词：急性病毒性坏死病毒 栉孔扇贝 浮游生物 病毒携带 荧光定量技术 

分 类 号：S944[农业科学—水产养殖]