鲤改良品系与体质量、体长性状相关的TRAP标记筛选  

Screening of TRAP markers associated with body weight and body length in an improved strain of common carp(Cyprinus carpio)

作　　者：董在杰[1,2,3] 曲疆奇[3] 袁新华[2,3] 苏胜彦[2] 谢庄[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081 [3]南京农业大学无锡渔业学院,江苏无锡214081

出　　处：《中国水产科学》2011年第4期801-808,共8页Journal of Fishery Sciences of China

基　　金：国家“十一五”科技支撑计划项目专题(2006BAD01A1208);现代农业产业技术体系建设专项资金项目(nycytx-49);农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放课题(BZ2009-06)

摘　　要：应用TRAP标记对一个鲤(Cyprinus carpio)改良品系72个个体进行扩增,采用其中25个多态性较好引物组合用于该群体遗传多样性分析。运用卡方检验分析25对引物在体质量差异显著的2组鱼中频率差异显著的位点,初步筛选出与体质量和体长性状相关的TRAP标记。将初步筛选的TRAP标记用于随机群体132个个体进行位点性状间的关联分析。结果表明,25个TRAP分子标记共扩增出353个位点,其中多态性位点230个,平均多态性比率65%,平均多态性信息含量为0.28,Nei’s遗传多样性指数平均值为0.219,Shannon信息指数平均值为0.329,表明该群体遗传多样性较为丰富。在初步筛选出的3个TRAP位点(gh1Trap04–140、4rTrap04–308、igf4Ga5–135)中,4rTrap04–308与体质量、体长呈极显著相关(P<0.01)。本研究利用TRAP这一新型分子标记对1个鲤改良群体进行遗传分析,并寻找出1个可能与鲤体质量,体长性状相关的功能基因位点,旨在为鲤体质量、体长性状的QTL定位和分子标记辅助育种奠定基础。Growth-related traits such as body weight,body length and condition factor are quantitative traits in-fluenced by a number of genes with relatively small effects,and environmental effects.These traits could be im-proved by phenotypic selection,but the genetic gain can be increased more rapidly with marker-assisted selection.Up to now,the genetic and molecular basis of growth-related traits is poorly understood in fish.A few QTL for growth-related traits have been detected with low density and fewer markers.Thus,more markers are needed to develop for mapping QTL.Recently,the newly developed TRAP（target region amplified polymorphism） marker is a reliable,stable and rapid PCR-based molecular marker technique.There are several advantages of TRAP over other DNA marker techniques.It has the favorable feature that combines high informativeness from the AFLP technique and easily performing from the RAPD method.TRAP markers use bioinformatics tools and EST data-base information to generate polymorphic markers around target gene sequences,while RAPD and AFLP are gen-erated by random anonymous sequences.TRAP maker has been effectively used in plant genetic research. In this study,the TRAP markers were used to analyze the genetic diversity and detect significant association between growth-related traits and polymorphism in an improved strain of common carp.The nucleotide sequences of three growth-related genes,including GH,GHR and IGF,were used to design fixed primer for pairing with four arbitrary primers.Finally,twenty-five combinations of primer were screened to be able to produce clear banding patterns and polymorphisms.The results showed that a total of 353 bands were scored with the 25 pairs of primer,of which 230 bands（65%） were polymorphism.The average of polymorphism information content（PIC）,Nei＇s gene diversity and Shannon＇s Information index was 0.28,0.219 3 and 0.328 9,respectively.Genotypic differences of the 25 TRAP markers were analyzed using χ2 test between the large body weight group and sma

关 键 词：鲤 TRAP标记 遗传多样性 体质量 体长 关联分析 

分 类 号：S917[农业科学—水产科学] Q953[生物学—动物学]