养殖圆斑星鲽血浆性类固醇激素表达与卵巢发育及温光调控的关系  

Annual gonadal maturation cycle of captive spotted halibut,Verasper variegatus:correlation with serum sex steroids and photothermal regulation

作　　者：徐永江[1,2] 柳学周[1,2] 王清印[1,2] 赵明[1,2] 王妍妍[1,2] 曲建忠 

机构地区：[1]农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,山东青岛266071 [2]中国水产科学研究院黄海水产研究所青岛市海水鱼类种子工程与生物技术重点实验室,山东青岛266071 [3]青岛忠海水产有限公司,山东青岛266414

出　　处：《中国水产科学》2011年第4期836-846,共11页Journal of Fishery Sciences of China

基　　金：国家863计划项目(2006AA10A4142010AA10A402);公益性农业行业专项项目(nyhyzx07-046);国家鲆鲽类产业技术体系建设项目(nycytx-50);农业科技成果转化资金项目(2008GB23260382)

摘　　要：系统研究了人工养殖条件下圆斑星鲽(Verasper variegatus)亲鱼血浆性类固醇激素的年周期变化规律及其与卵巢发育成熟及温光调控的关系。结果表明,圆斑星鲽卵巢发育属非同步分批发育模式,一年一次成熟分批产卵类型。卵巢年周期发育过程中可见5个发育时相的卵母细胞,卵巢发育可化分为4个成熟阶段,6个发育时期。性腺指数(GSI)、肝脏指数(HSI)和肥满度(CF)在亲鱼的年周期发育中呈现规律性的变化。亲鱼血浆中雌二醇(E2)在产卵期达峰值;排卵结束后,E2表达水平降至较低水平。血浆中睾酮(T)含量在10月达峰值,并在产卵期间保持相对较高表达水平。统计分析表明,血浆E2水平与GSI和HSI值的变化都呈现显著的正相关关系(P<0.05)。水温和光周期对卵巢发育具有明显的影响并显著影响E2的表达,GSI与水温存在显著的负相关关系(P<0.05),HSI与水温存在显著的负相关关系(P<0.05),而CF与光周期呈现显著的负相关关系(P<0.05)。结论认为,圆斑星鲽雌性亲鱼血浆性类固醇激素表达水平变化与性腺发育(卵母细胞成熟)、水温等具有显著的相关关系。本研究结果可作为温光调控人工亲鱼性腺发育成熟、激素诱导亲鱼产卵的重要参考技术依据。We documented the annual ovarian maturation cycle and measured serum sex steroids levels in the spotted halibut,Verasper variegtus,using histological and morphometric methods and radioimmunoassay.In addi-tion,we analyzed the relationship between the gonadosomatic index（GSI）,condition factor（CF）,hepatosomatic index（HSI）,sex steroid levels and temperature or photoperiod.Oocyte development was asynchronous and the halibut exhibited batch spawning behavior.We divided oocyte growth in sexually mature females into five phases.Similarly,the ovarian maturation cycle was divided into four stages.GSI,HSI,and CF all increased prior to,or during the peak phase of vitellogenic growth corresponding to gonad development.The GSI was positively corre-lated with HSI and CF.Temperature had a greater influence on gonad maturation（GSI and HSI） than photoperiod.The level of serum estradiol began to increase in November and peaked in January,thereafter decreasing to a rela-tively low level until the following reproductive cycle.The level of serum testosterone peaked in October,two months earlier than that of the peak in estradiol.We found a positive relationship between the levels of estradiol and testosterone.Both temperature and photoperiod had a significant positive effect on levels of serum estradiol,which was also positively correlated with GSI and HSI.In addition,temperature and photoperiod had a significant effect on HSI and CF,respectively.Our results provide insight into the physiological and endocrine mechanisms controlling the annual reproductive cycle of captive female spotted halibut brood.In addition,our observations provide guidance for broodstock programs and for the determining the optimal timing for hormone induced spawning.

关 键 词：圆斑星鲽 卵巢发育 性类固醇激素 温光调控 

分 类 号：S96[农业科学—水产养殖]