多效价载体疫苗免疫大菱鲆效果评价  

Evaluation of immune effects of a multivalent vector vaccine in turbot,Scophthalmus maximus

作　　者：王秀华[1] 周凌云[2] 王玉娟[1] 刘琴[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]华东理工大学,上海200237

出　　处：《中国水产科学》2011年第4期918-923,共6页Journal of Fishery Sciences of China

基　　金：国家"863"计划项目(2006AA100310);国家科技支撑计划项目(2006BAD03B0504);山东省科技攻关计划项目(2006GG2205005);基本科研业务费专项资金资助项目(2007-GY-03)

摘　　要：采用注射与浸泡2种方法,对构建的多效价载体疫苗MVAV6203A-1进行了免疫保护效果和血清效价的研究。结果显示,用多效价载体疫苗注射免疫大菱鲆(Scophthalmus maximus),可同时获得对鳗弧菌(Vibrio anguil-larum)和嗜水气单胞菌(Aeromonas hydrophila)的免疫保护效果,免疫相对保护率分别为80.6%和77.0%;用ELISA方法测定血清效价的结果显示,免疫MVAV6203A-1后大菱鲆血清效价最高达到2 048,均值为891,明显高于对照组的97;用多效价载体疫苗浸泡免疫大菱鲆后,对鳗弧菌与嗜水气单胞菌的免疫相对保护率分别为62.2%和11.7%。血清效价分析显示,实验组与对照组的均值均为64。上述结果表明,所构建的疫苗MVAV6203A-1可有效提高大菱鲆对鳗弧菌和嗜水气单胞菌的免疫力,并且免疫和攻毒的途径可能是影响免疫保护效果的因子。本研究旨在为鳗弧菌与嗜水气单胞菌引起的鱼类疾病防治提供技术支撑。Vibrio anguillarum and Aeromonas hydrophila are the primary cause of mortality in marine and fresh water aquaculture.Mortality,due to disease from these two pathogens,has resulted in significant economic loss.Historically,infected fish have been treated with antibiotics,leading to widespread drug resistance among patho-genic bacteria.More recently,a number of vaccines against pathogenic bacteria have been developed and ap-proved for use in aquaculture.Although China has a long history of intensive fish culture,there has been little progress in developing/using vaccines for the treatment of fish diseases in this region.Our objective was to de-velop a combined,single application vaccine against V.anguillarum and A.hydrophila.We constructed a multi-valent vector vaccine（MVAV6203A-1） by fusing glyceraldehyde-3-phosphate dehydrogenase（GAPDH） from pathogenic A.hydrophila to attenuated V.anguillarum.We then evaluated the effectiveness of this vaccine against the two pathogens.Turbot,body length（8.99±0.73） cm,were immunized with the vaccine（MVAV6203A-1） by intraperitoneal injection（i.p.） or immersion.The fish were then challenged with virulent strain V.anguillarum MVM425 and A.hydrophila LSA34 by i.p injection and held for 30 d.We measured the relative percent survival（RPS） and antibody titers in the plasma against V.anguillarum by indirect ELISA at the end of the experiment.Fish that were given the vaccine by i.p.injection had high protections against MVM425 and LSA34 with RPS of 80.6% and 77.0% respectively.Similarly,the fish that were immersed in the vaccine also had a protection against MVM425（RPS 62.2%）,but had a low protection against LSA34（RPS 11.7%）.The plasma antibody titers were higher in the i.p.vaccination group（max： 2048,mean： 891） than in the control（mean： 97）.Conversely,there was no difference in the mean antibody titer between the immersion treatment group and the control（mean： 64）.Thus,our data suggest that the multivalent vector vaccine MVAV6203A-1 is

关 键 词：多效价载体疫苗 大菱鲆 鳗弧菌 嗜水气单胞菌 免疫保护率 抗体效价 

分 类 号：S96[农业科学—水产养殖]