纳米粒子表面修饰对其与细胞膜相互作用的影响  

A Theoretical Study of Surface Modification Effect on the Interaction between Nanoparticles and Cell Membrane

作　　者：关映雪[1] 张爱丽[1] 

机构地区：[1]上海交通大学生物医学工程学院,上海200240

出　　处：《中国科学：生命科学》2011年第7期558-564,共7页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:NSFC51076095;NSFC50725622);国家重点基础研究发展计划(批准号:2009CB930403);上海市科委启明星人才计划(批准号:10QA1403900)资助项目

摘　　要：纳米粒子在生物医学上的应用越来越广泛,其进入细胞的机制与规律是设计与开发的基础.已有研究发现,表面修饰不同亲疏水性基团的金纳米粒子,在内吞机制被抑制时,进入细胞的能力明显不同.更特别的是,粒子表面亲水性基团与疏水性基团呈间隔条纹规则排列的纳米粒子,与其他修饰成分相同仅排列不同的纳米粒子进入细胞的规律区别显著.这一特殊现象无法用已有的纳米粒子进入细胞的机制解释.本文针对该研究结果,将纳米粒子与细胞的体系简化,定量分析了3种不同纳米粒子进入细胞前后的不同状态,计算获得了表面修饰不同亲疏水性基团的纳米粒子与细胞膜之间相互作用的Flory-Huggins自由能.结果发现,修饰规则间隔排列亲疏水基团的纳米粒子,其作用自由能在与细胞接触前后变化最大.研究结果不仅解释了实验发现,同时预示了纳米粒子进入细胞的新机制.Applications of nanoparticles in biology and medicine are getting increasingly popular. The mechanisms of their intrusion across the cell membrane are key issues for specific nanoparticle design and application. Recent experimental report has shown that there exists a new passive cellular uptake mechanism of nanoparticles. In this study, a model is developed to quantify a possible driving force for the nanoparticle intrusion into the cell by simplifying the interaction system. The Flory-Huggins interaction free energies are calculated between the cell membrane and the differently-coated nanoparticles. Differences are found in free energies of the system in different states. The results can be used to interpret the newly reported experimental observations.

关 键 词：Flory—Huggins自由能 细胞膜 纳米粒子 亲疏水基团 溶度参数 

分 类 号：TB383.1[一般工业技术—材料科学与工程]