Cloning of Broad-spectrum Anti-disease NPR1 Gene with RT-PCR and Construction of Its Protein Expression Vector  

RT-PCR克隆广谱抗病基因NPR1及其蛋白表达载体构建(英文)

作　　者：刘永光[1,2] 刘克锋[2] 孙向阳[1] 

机构地区：[1]北京林业大学水保学院,北京100083 [2]北京农学院城乡发展学院,北京102206

出　　处：《Agricultural Science & Technology》2011年第6期852-854,930,共4页农业科学与技术（英文版）

基　　金：Supported by project of Beijing Municipal Education Commission(KM200910020014);Project of Sand Control Department,Beijing Municipal Landscape Greening Bureau(2008)~~

摘　　要：[Objective] It is to clone broad-spectrum anti-disease gene NPR1 and to construct its protein expression vector.[Method] First to extract total RNA of Arabidopsis thaliana and design relevant primers,and then the method of reverse transcription PCR was adopted to clone.With the method of enzyme digestion and ligation,this gene will be directed into protein expression vector.[Result] After relevant testing,NPR1 was inserted into vector pMXB10 to obtain pMXB10-NPR1 protein expression vector.[Conclusion] Protein expression vector including NPR1 was successfully constructed.[目的]克隆植物广谱抗病基因NPR1并构建其蛋白表达载体。[方法]提取拟南芥总RNA,设计相关引物,采用反转录PCR方法克隆NPR1基因;利用酶切连接方法,将该基因正向导入蛋白表达载体。[结果]经过相关检验,将NPR1正向插入pMXB10载体中,得到了pMXB10-NPR1蛋白表达载体。[结论]成功构建了包含NPR1的蛋白表达载体。

关 键 词：Nonexpressor of pathogenesis-related genes 1（NPR1） Broad-spectrum anti-disease Construction of vectors 

分 类 号：S188[农业科学—农业基础科学]