Experimental Research of Acupuncture Therapy on Skin Aging in Senile Rats  

针刺对老龄大鼠皮肤老化影响的实验研究(英文)

作　　者：芦源 任宝琴 张鸥 王臻 

机构地区：[1]Affiliated Hospital of Liaoning University of Traditional Chinese Medicine [2]Liaoning University of Traditional Chinese Medicine

出　　处：《Journal of Acupuncture and Tuina Science》2011年第3期137-141,共5页针灸推拿医学（英文版）

摘　　要：Objective： To observe the effect of acupuncture on skin aging of senile rats and discuss the mechanism of acupuncture in treating skin aging. Methods： The 18-month-old senile Wistar rats were randomly divided into three groups, a blank group, a surrounding needling group, a surrounding needling plus Zusanli （ST 36） group, comparing with the 8-month-old young rats. The tests included the content of dissoluble hydroxyproline （Hyp） and the activity of superoxide dismutase （SOD） in the skin of rats＇ abdomen. The transmission electron microscope was used for the morphological observation of the fibroblasts and the arrangement of the collagen fiber. Results： The dermal organelles in the fibroblasts of the rats decreased with the cellular structures. Meanwhile, the spaces among the collagen fibers extended and the aging bridging increased. The surrounding needling improved the activity of the fibroblasts. The dissoluble Hyp content in the surrounding needling group was much higher than the rats in the blank group, but the SOD activity didn＇t change significantly. However, the surrounding needling plus Zusanli （ST 36） enhanced the SOD activity. Conclusion： Acupuncture may improve the activity of the fibroblasts in the skin to increase the dissoluble collagen content, resulting in the improvement of an aged appearance. Surrounding needling plus needling Zusanli （ST 36） could enhance the SOD activity.目的:观察针刺对老龄大鼠皮肤老化的影响,探讨针刺抗皮肤老化的作用机理。方法:选用18月龄老年大鼠为研究对象,随机分为3组,围刺组、围刺加足三里组、空白组,并与8月龄青年大鼠对照,测定各组大鼠腹部皮肤的可溶性羟脯氨酸(Hydroxyproline,Hyp)含量与超氧化物歧化酶(Superoxide Dismutase,SOD)活性,并应用透射电镜对比观察成纤维细胞的形态以及胶原纤维的排列情况。结果:老龄大鼠真皮成纤维细胞的细胞器减少,细胞结构退化,同时胶原纤维之间空隙加宽,老化性架桥增多。通过围刺治疗,可以使其成纤维细胞活性增强。同时,围刺治疗后老年大鼠皮肤的可溶性羟脯氨酸含量较空白组明显增加,SOD活性无显著变化,而围刺加足三里组皮肤组织中SOD活性增强。结论:针刺可能是通过增强皮肤中成纤维细胞的活性进而增加皮肤中可溶性胶原含量来改变皮肤的老化外观。配合足三里穴可以增加皮肤的SOD活性。

关 键 词：Acupuncture Therapy Points  ST 36 （Zusanli） Skin Aging HYDROXYPROLINE SUPEROXIDEDISMUTASE RATS 

分 类 号：R2-03[医药卫生—中医学]